Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1985c

Experiment: 7H9 with Chlorpromazine 0.054 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1984c and Rv1985c are separated by 429 nucleotidesRv1985c and Rv1986 are separated by 108 nucleotides Rv1984c: Rv1984c - Probable cutinase precursor CFP21, at 2,227,908 to 2,228,561 Rv1984c Rv1985c: Rv1985c - Probable transcriptional regulatory protein (probably LysR-family), at 2,228,991 to 2,229,902 Rv1985c Rv1986: Rv1986 - Probable conserved integral membrane protein, at 2,230,011 to 2,230,610 Rv1986 Position (kb) 2228 2229 2230Strain fitness (log2 ratio) -2 -1 0 1 2at 2227.991 kb on + strand, within Rv1984cat 2227.992 kb on - strand, within Rv1984cat 2227.992 kb on - strand, within Rv1984cat 2227.992 kb on - strand, within Rv1984cat 2227.994 kb on + strand, within Rv1984cat 2227.994 kb on + strand, within Rv1984cat 2227.994 kb on + strand, within Rv1984cat 2228.013 kb on + strand, within Rv1984cat 2228.014 kb on - strand, within Rv1984cat 2228.048 kb on + strand, within Rv1984cat 2228.054 kb on + strand, within Rv1984cat 2228.054 kb on + strand, within Rv1984cat 2228.055 kb on - strand, within Rv1984cat 2228.055 kb on - strand, within Rv1984cat 2228.213 kb on - strand, within Rv1984cat 2228.309 kb on - strand, within Rv1984cat 2228.326 kb on + strand, within Rv1984cat 2228.327 kb on - strand, within Rv1984cat 2228.327 kb on - strand, within Rv1984cat 2228.372 kb on + strand, within Rv1984cat 2228.372 kb on + strand, within Rv1984cat 2228.373 kb on - strand, within Rv1984cat 2228.373 kb on - strand, within Rv1984cat 2228.577 kb on + strandat 2228.577 kb on + strandat 2228.664 kb on + strandat 2228.760 kb on - strandat 2228.760 kb on - strandat 2228.763 kb on + strandat 2228.764 kb on - strandat 2228.905 kb on - strandat 2228.905 kb on - strandat 2228.966 kb on + strandat 2229.103 kb on + strand, within Rv1985cat 2229.123 kb on + strand, within Rv1985cat 2229.123 kb on + strand, within Rv1985cat 2229.124 kb on - strand, within Rv1985cat 2229.405 kb on + strand, within Rv1985cat 2229.406 kb on - strand, within Rv1985cat 2229.406 kb on - strand, within Rv1985cat 2229.406 kb on - strand, within Rv1985cat 2229.601 kb on - strand, within Rv1985cat 2229.707 kb on + strand, within Rv1985cat 2229.708 kb on - strand, within Rv1985cat 2229.708 kb on - strand, within Rv1985cat 2229.708 kb on - strand, within Rv1985cat 2229.813 kb on + strandat 2229.814 kb on - strandat 2229.814 kb on - strandat 2229.814 kb on - strandat 2230.384 kb on - strand, within Rv1986at 2230.384 kb on - strand, within Rv1986at 2230.478 kb on - strand, within Rv1986at 2230.608 kb on + strandat 2230.611 kb on + strandat 2230.611 kb on + strandat 2230.612 kb on - strandat 2230.612 kb on - strandat 2230.662 kb on + strandat 2230.662 kb on + strandat 2230.663 kb on - strandat 2230.663 kb on - strandat 2230.783 kb on - strandat 2230.803 kb on - strandat 2230.839 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chlorpromazine 0.054 mM
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2,227,991 + Rv1984c 0.13 +0.5
2,227,992 - Rv1984c 0.13 +0.0
2,227,992 - Rv1984c 0.13 -0.5
2,227,992 - Rv1984c 0.13 -0.4
2,227,994 + Rv1984c 0.13 +0.7
2,227,994 + Rv1984c 0.13 -1.2
2,227,994 + Rv1984c 0.13 -0.3
2,228,013 + Rv1984c 0.16 +0.1
2,228,014 - Rv1984c 0.16 +2.3
2,228,048 + Rv1984c 0.21 +0.4
2,228,054 + Rv1984c 0.22 +0.0
2,228,054 + Rv1984c 0.22 -0.3
2,228,055 - Rv1984c 0.22 -0.9
2,228,055 - Rv1984c 0.22 -0.1
2,228,213 - Rv1984c 0.47 +0.3
2,228,309 - Rv1984c 0.61 +0.3
2,228,326 + Rv1984c 0.64 +0.1
2,228,327 - Rv1984c 0.64 +0.7
2,228,327 - Rv1984c 0.64 -1.0
2,228,372 + Rv1984c 0.71 -0.2
2,228,372 + Rv1984c 0.71 +0.4
2,228,373 - Rv1984c 0.71 +0.4
2,228,373 - Rv1984c 0.71 +1.9
2,228,577 + +0.2
2,228,577 + -1.2
2,228,664 + -0.2
2,228,760 - +1.3
2,228,760 - +1.2
2,228,763 + -0.4
2,228,764 - +2.6
2,228,905 - -0.3
2,228,905 - -0.2
2,228,966 + -0.7
2,229,103 + Rv1985c 0.12 +0.0
2,229,123 + Rv1985c 0.14 -0.2
2,229,123 + Rv1985c 0.14 +0.2
2,229,124 - Rv1985c 0.15 +1.0
2,229,405 + Rv1985c 0.45 -0.8
2,229,406 - Rv1985c 0.46 -1.1
2,229,406 - Rv1985c 0.46 -2.5
2,229,406 - Rv1985c 0.46 -0.1
2,229,601 - Rv1985c 0.67 -1.4
2,229,707 + Rv1985c 0.79 -0.3
2,229,708 - Rv1985c 0.79 -1.2
2,229,708 - Rv1985c 0.79 -0.7
2,229,708 - Rv1985c 0.79 -0.5
2,229,813 + -0.5
2,229,814 - -0.7
2,229,814 - +0.6
2,229,814 - -0.7
2,230,384 - Rv1986 0.62 -0.4
2,230,384 - Rv1986 0.62 -0.0
2,230,478 - Rv1986 0.78 -0.9
2,230,608 + +0.0
2,230,611 + +0.2
2,230,611 + -0.2
2,230,612 - +2.2
2,230,612 - +0.1
2,230,662 + -0.4
2,230,662 + -0.3
2,230,663 - -0.1
2,230,663 - -1.8
2,230,783 - -0.2
2,230,803 - -0.3
2,230,839 + +0.4

Or see this region's nucleotide sequence