Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2003c

Experiment: 7H9 with Erythromycin 0.016 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2001 and Rv2002 are separated by 75 nucleotidesRv2002 and Rv2003c are separated by 120 nucleotidesRv2003c and Rv2004c are separated by 57 nucleotides Rv2001: Rv2001 - Conserved hypothetical protein, at 2,246,832 to 2,247,584 Rv2001 Rv2002: Rv2002 - Possible 20-beta-hydroxysteroid dehydrogenase FabG3 (cortisone reductase) ((R)-20-hydroxysteroid dehydrogenase), at 2,247,660 to 2,248,442 Rv2002 Rv2003c: Rv2003c - Conserved hypothetical protein, at 2,248,563 to 2,249,420 Rv2003c Rv2004c: Rv2004c - Conserved protein, at 2,249,478 to 2,250,974 Rv2004c Position (kb) 2248 2249 2250Strain fitness (log2 ratio) -2 -1 0 1at 2247.580 kb on + strandat 2247.580 kb on + strandat 2247.713 kb on + strandat 2248.376 kb on - strandat 2248.440 kb on + strandat 2248.565 kb on - strandat 2248.593 kb on - strandat 2248.771 kb on + strand, within Rv2003cat 2248.823 kb on - strand, within Rv2003cat 2249.326 kb on - strand, within Rv2003cat 2249.504 kb on - strandat 2249.880 kb on + strand, within Rv2004cat 2250.140 kb on - strand, within Rv2004c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Erythromycin 0.016 mM
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2,247,580 + +0.9
2,247,580 + -0.2
2,247,713 + -0.9
2,248,376 - -0.9
2,248,440 + +0.1
2,248,565 - -0.2
2,248,593 - -0.4
2,248,771 + Rv2003c 0.24 -1.9
2,248,823 - Rv2003c 0.30 -0.6
2,249,326 - Rv2003c 0.89 -0.8
2,249,504 - -0.5
2,249,880 + Rv2004c 0.27 -1.2
2,250,140 - Rv2004c 0.44 -0.8

Or see this region's nucleotide sequence