Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2223c

Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.0012 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2222c and Rv2223c are separated by 94 nucleotidesRv2223c and Rv2224c are separated by 61 nucleotides Rv2222c: Rv2222c - Probable glutamine synthetase GlnA2 (glutamine synthase) (GS-II), at 2,492,402 to 2,493,742 Rv2222c Rv2223c: Rv2223c - Probable exported protease, at 2,493,837 to 2,495,399 Rv2223c Rv2224c: Rv2224c - Probable carboxylesterase CaeA, at 2,495,461 to 2,497,023 Rv2224c Position (kb) 2493 2494 2495 2496Strain fitness (log2 ratio) -2 -1 0 1at 2492.856 kb on + strand, within Rv2222cat 2493.252 kb on - strand, within Rv2222cat 2493.360 kb on + strand, within Rv2222cat 2493.754 kb on + strandat 2493.990 kb on + strandat 2494.003 kb on + strand, within Rv2223cat 2494.149 kb on - strand, within Rv2223cat 2494.149 kb on - strand, within Rv2223cat 2494.149 kb on - strand, within Rv2223cat 2494.291 kb on + strand, within Rv2223cat 2494.291 kb on + strand, within Rv2223cat 2494.292 kb on - strand, within Rv2223cat 2494.292 kb on - strand, within Rv2223cat 2494.292 kb on - strand, within Rv2223cat 2494.384 kb on + strand, within Rv2223cat 2494.385 kb on - strand, within Rv2223cat 2494.420 kb on + strand, within Rv2223cat 2494.420 kb on + strand, within Rv2223cat 2494.421 kb on - strand, within Rv2223cat 2494.487 kb on - strand, within Rv2223cat 2494.555 kb on + strand, within Rv2223cat 2494.555 kb on + strand, within Rv2223cat 2494.556 kb on - strand, within Rv2223cat 2494.629 kb on - strand, within Rv2223cat 2494.662 kb on - strand, within Rv2223cat 2494.703 kb on - strand, within Rv2223cat 2494.703 kb on - strand, within Rv2223cat 2494.703 kb on - strand, within Rv2223cat 2494.717 kb on + strand, within Rv2223cat 2494.717 kb on + strand, within Rv2223cat 2494.717 kb on + strand, within Rv2223cat 2495.028 kb on - strand, within Rv2223cat 2495.155 kb on + strand, within Rv2223cat 2495.156 kb on - strand, within Rv2223cat 2495.322 kb on + strandat 2495.415 kb on + strandat 2495.415 kb on + strandat 2495.416 kb on - strandat 2495.504 kb on + strandat 2495.545 kb on + strandat 2495.545 kb on + strandat 2495.546 kb on - strandat 2495.546 kb on - strandat 2495.621 kb on + strand, within Rv2224cat 2495.622 kb on - strand, within Rv2224cat 2495.622 kb on - strand, within Rv2224cat 2495.622 kb on - strand, within Rv2224cat 2495.762 kb on + strand, within Rv2224cat 2495.763 kb on - strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.999 kb on + strand, within Rv2224cat 2496.000 kb on - strand, within Rv2224cat 2496.010 kb on - strand, within Rv2224cat 2496.033 kb on + strand, within Rv2224cat 2496.033 kb on + strand, within Rv2224cat 2496.034 kb on - strand, within Rv2224cat 2496.035 kb on + strand, within Rv2224cat 2496.036 kb on - strand, within Rv2224cat 2496.123 kb on - strand, within Rv2224cat 2496.191 kb on + strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.315 kb on - strand, within Rv2224cat 2496.315 kb on - strand, within Rv2224cat 2496.339 kb on - strand, within Rv2224cat 2496.345 kb on - strand, within Rv2224cat 2496.345 kb on - strand, within Rv2224cat 2496.353 kb on + strand, within Rv2224c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.0012 mM
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2,492,856 + Rv2222c 0.34 -0.2
2,493,252 - Rv2222c 0.63 -1.1
2,493,360 + Rv2222c 0.71 -0.5
2,493,754 + +0.9
2,493,990 + +0.4
2,494,003 + Rv2223c 0.11 -0.2
2,494,149 - Rv2223c 0.20 +0.2
2,494,149 - Rv2223c 0.20 -0.6
2,494,149 - Rv2223c 0.20 -0.1
2,494,291 + Rv2223c 0.29 -1.2
2,494,291 + Rv2223c 0.29 +0.2
2,494,292 - Rv2223c 0.29 -0.5
2,494,292 - Rv2223c 0.29 -0.8
2,494,292 - Rv2223c 0.29 -0.9
2,494,384 + Rv2223c 0.35 -0.3
2,494,385 - Rv2223c 0.35 +0.3
2,494,420 + Rv2223c 0.37 -0.6
2,494,420 + Rv2223c 0.37 +0.2
2,494,421 - Rv2223c 0.37 +0.1
2,494,487 - Rv2223c 0.42 -0.7
2,494,555 + Rv2223c 0.46 -0.1
2,494,555 + Rv2223c 0.46 -1.7
2,494,556 - Rv2223c 0.46 +0.2
2,494,629 - Rv2223c 0.51 -0.7
2,494,662 - Rv2223c 0.53 -0.4
2,494,703 - Rv2223c 0.55 -0.4
2,494,703 - Rv2223c 0.55 -0.2
2,494,703 - Rv2223c 0.55 +0.6
2,494,717 + Rv2223c 0.56 -0.4
2,494,717 + Rv2223c 0.56 +0.1
2,494,717 + Rv2223c 0.56 +0.8
2,495,028 - Rv2223c 0.76 -2.5
2,495,155 + Rv2223c 0.84 -0.0
2,495,156 - Rv2223c 0.84 -0.2
2,495,322 + -0.9
2,495,415 + -0.1
2,495,415 + +0.2
2,495,416 - -0.2
2,495,504 + +0.1
2,495,545 + +0.2
2,495,545 + +0.2
2,495,546 - -2.1
2,495,546 - -0.7
2,495,621 + Rv2224c 0.10 -0.9
2,495,622 - Rv2224c 0.10 +1.3
2,495,622 - Rv2224c 0.10 -0.9
2,495,622 - Rv2224c 0.10 -1.5
2,495,762 + Rv2224c 0.19 -0.8
2,495,763 - Rv2224c 0.19 -1.1
2,495,909 + Rv2224c 0.29 -0.6
2,495,909 + Rv2224c 0.29 -0.7
2,495,909 + Rv2224c 0.29 -0.5
2,495,909 + Rv2224c 0.29 -1.7
2,495,909 + Rv2224c 0.29 -0.8
2,495,909 + Rv2224c 0.29 -0.8
2,495,910 - Rv2224c 0.29 -0.8
2,495,910 - Rv2224c 0.29 -0.9
2,495,910 - Rv2224c 0.29 -0.9
2,495,910 - Rv2224c 0.29 +0.4
2,495,999 + Rv2224c 0.34 +0.6
2,496,000 - Rv2224c 0.34 -0.5
2,496,010 - Rv2224c 0.35 -0.4
2,496,033 + Rv2224c 0.37 -0.8
2,496,033 + Rv2224c 0.37 -0.6
2,496,034 - Rv2224c 0.37 -0.5
2,496,035 + Rv2224c 0.37 -0.4
2,496,036 - Rv2224c 0.37 -1.0
2,496,123 - Rv2224c 0.42 +0.3
2,496,191 + Rv2224c 0.47 +0.2
2,496,192 - Rv2224c 0.47 -0.3
2,496,192 - Rv2224c 0.47 +0.5
2,496,192 - Rv2224c 0.47 +0.5
2,496,192 - Rv2224c 0.47 -1.9
2,496,315 - Rv2224c 0.55 -0.9
2,496,315 - Rv2224c 0.55 +1.2
2,496,339 - Rv2224c 0.56 -1.0
2,496,345 - Rv2224c 0.57 -0.8
2,496,345 - Rv2224c 0.57 -0.8
2,496,353 + Rv2224c 0.57 -0.6

Or see this region's nucleotide sequence