Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0021c

Experiment: 7H9 with Ciprofloxacin Hydrochloride 0.0012 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0020c and GFF23 are separated by 199 nucleotidesGFF23 and Rv0021c are separated by 186 nucleotidesRv0021c and Rv0022c are separated by 141 nucleotidesRv0022c and Rv0023 are separated by 152 nucleotides Rv0020c: Rv0020c - Conserved protein with FHA domain, FhaA, at 23,861 to 25,444 Rv0020c GFF23 - tRNA-Leu, at 25,644 to 25,726 GFF23 Rv0021c: Rv0021c - Conserved hypothetical protein, at 25,913 to 26,881 Rv0021c Rv0022c: Rv0022c - Probable transcriptional regulatory protein WhiB-like WhiB5, at 27,023 to 27,442 Rv0022c Rv0023: Rv0023 - Possible transcriptional regulatory protein, at 27,595 to 28,365 Rv0023 Position (kb) 25 26 27Strain fitness (log2 ratio) -2 -1 0 1 2 3at 24.987 kb on - strand, within Rv0020cat 24.987 kb on - strand, within Rv0020cat 25.143 kb on + strand, within Rv0020cat 25.170 kb on + strand, within Rv0020cat 25.171 kb on - strand, within Rv0020cat 25.171 kb on - strand, within Rv0020cat 25.260 kb on + strand, within Rv0020cat 25.261 kb on - strand, within Rv0020cat 25.261 kb on - strand, within Rv0020cat 25.261 kb on - strand, within Rv0020cat 25.261 kb on - strand, within Rv0020cat 25.505 kb on + strandat 25.505 kb on + strandat 25.506 kb on - strandat 25.506 kb on - strandat 25.616 kb on + strandat 25.616 kb on + strandat 25.616 kb on + strandat 25.616 kb on + strandat 25.616 kb on + strandat 25.617 kb on - strandat 25.617 kb on - strandat 25.624 kb on + strandat 25.730 kb on + strandat 25.730 kb on + strandat 25.731 kb on - strandat 25.796 kb on + strandat 25.907 kb on - strandat 25.914 kb on + strandat 25.915 kb on - strandat 25.915 kb on - strandat 25.915 kb on - strandat 25.915 kb on - strandat 25.960 kb on + strandat 26.040 kb on + strand, within Rv0021cat 26.040 kb on + strand, within Rv0021cat 26.040 kb on + strand, within Rv0021cat 26.041 kb on - strand, within Rv0021cat 26.041 kb on - strand, within Rv0021cat 26.068 kb on - strand, within Rv0021cat 26.141 kb on + strand, within Rv0021cat 26.141 kb on + strand, within Rv0021cat 26.142 kb on - strand, within Rv0021cat 26.193 kb on + strand, within Rv0021cat 26.193 kb on + strand, within Rv0021cat 26.197 kb on + strand, within Rv0021cat 26.197 kb on + strand, within Rv0021cat 26.197 kb on + strand, within Rv0021cat 26.197 kb on + strand, within Rv0021cat 26.197 kb on + strand, within Rv0021cat 26.198 kb on - strand, within Rv0021cat 26.291 kb on - strand, within Rv0021cat 26.622 kb on + strand, within Rv0021cat 26.623 kb on - strand, within Rv0021cat 26.623 kb on - strand, within Rv0021cat 26.623 kb on - strand, within Rv0021cat 26.780 kb on + strand, within Rv0021cat 26.861 kb on - strandat 26.933 kb on - strandat 26.933 kb on - strandat 26.933 kb on - strandat 27.214 kb on - strand, within Rv0022cat 27.245 kb on + strand, within Rv0022cat 27.253 kb on - strand, within Rv0022cat 27.253 kb on - strand, within Rv0022cat 27.299 kb on + strand, within Rv0022cat 27.300 kb on - strand, within Rv0022cat 27.410 kb on + strandat 27.410 kb on + strandat 27.410 kb on + strandat 27.411 kb on - strandat 27.411 kb on - strandat 27.411 kb on - strandat 27.411 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ciprofloxacin Hydrochloride 0.0012 mM
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24,987 - Rv0020c 0.71 -0.6
24,987 - Rv0020c 0.71 -0.7
25,143 + Rv0020c 0.81 -0.5
25,170 + Rv0020c 0.83 +0.3
25,171 - Rv0020c 0.83 -0.6
25,171 - Rv0020c 0.83 -0.3
25,260 + Rv0020c 0.88 -1.0
25,261 - Rv0020c 0.88 -1.1
25,261 - Rv0020c 0.88 -0.4
25,261 - Rv0020c 0.88 +0.2
25,261 - Rv0020c 0.88 +1.4
25,505 + -1.8
25,505 + -0.7
25,506 - -0.1
25,506 - -0.8
25,616 + +0.3
25,616 + -0.2
25,616 + +1.2
25,616 + +0.3
25,616 + -0.1
25,617 - -0.1
25,617 - -0.9
25,624 + +1.4
25,730 + -0.4
25,730 + +0.5
25,731 - -1.1
25,796 + +0.3
25,907 - -0.8
25,914 + -1.1
25,915 - -0.1
25,915 - -0.6
25,915 - +0.3
25,915 - -0.1
25,960 + -1.1
26,040 + Rv0021c 0.13 +2.9
26,040 + Rv0021c 0.13 -0.2
26,040 + Rv0021c 0.13 +1.4
26,041 - Rv0021c 0.13 -0.3
26,041 - Rv0021c 0.13 -0.1
26,068 - Rv0021c 0.16 +0.5
26,141 + Rv0021c 0.24 +0.0
26,141 + Rv0021c 0.24 -0.0
26,142 - Rv0021c 0.24 +1.3
26,193 + Rv0021c 0.29 -0.3
26,193 + Rv0021c 0.29 -0.7
26,197 + Rv0021c 0.29 -0.8
26,197 + Rv0021c 0.29 +0.1
26,197 + Rv0021c 0.29 -0.7
26,197 + Rv0021c 0.29 +0.0
26,197 + Rv0021c 0.29 +0.6
26,198 - Rv0021c 0.29 +0.0
26,291 - Rv0021c 0.39 -0.7
26,622 + Rv0021c 0.73 +0.4
26,623 - Rv0021c 0.73 -0.7
26,623 - Rv0021c 0.73 +0.2
26,623 - Rv0021c 0.73 -0.1
26,780 + Rv0021c 0.89 -0.1
26,861 - +0.4
26,933 - +0.4
26,933 - -0.4
26,933 - -0.1
27,214 - Rv0022c 0.45 -0.8
27,245 + Rv0022c 0.53 +0.2
27,253 - Rv0022c 0.55 -0.4
27,253 - Rv0022c 0.55 -0.1
27,299 + Rv0022c 0.66 -0.0
27,300 - Rv0022c 0.66 -0.2
27,410 + -0.6
27,410 + -0.2
27,410 + -0.6
27,411 - -0.5
27,411 - +0.1
27,411 - +1.4
27,411 - +0.4

Or see this region's nucleotide sequence