Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3605c

Experiment: 7H9 with Chlorhexidine diacetate salt 0.0021 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3604c and Rv3605c are separated by 208 nucleotidesRv3605c and Rv3606c overlap by 1 nucleotidesRv3606c and Rv3607c overlap by 4 nucleotidesRv3607c and Rv3608c overlap by 8 nucleotides Rv3604c: Rv3604c - Probable conserved transmembrane protein rich in alanine and arginine and proline, at 4,046,303 to 4,047,496 Rv3604c Rv3605c: Rv3605c - Probable conserved secreted protein, at 4,047,705 to 4,048,181 Rv3605c Rv3606c: Rv3606c - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridinepyrophosphokinase FolK (7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase) (HPPK) (6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase) (PPPK) (2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase) (7,8-dihydro-6-hydroxymethylpterin-diphosphokinase) (6-hydroxymethyl-7,8-dihydropterin diphosphokinase), at 4,048,181 to 4,048,747 Rv3606c Rv3607c: Rv3607c - Probable dihydroneopterin aldolase FolB (DHNA), at 4,048,744 to 4,049,145 Rv3607c Rv3608c: Rv3608c - Dihydropteroate synthase 1 FolP (DHPS 1) (dihydropteroate pyrophosphorylase 1) (dihydropteroate diphosphorylase 1), at 4,049,138 to 4,049,980 Rv3608c Position (kb) 4047 4048 4049Strain fitness (log2 ratio) -1 0 1at 4047.596 kb on + strandat 4047.872 kb on - strand, within Rv3605cat 4047.933 kb on + strand, within Rv3605cat 4048.018 kb on + strand, within Rv3605cat 4048.120 kb on + strand, within Rv3605cat 4048.120 kb on + strand, within Rv3605cat 4048.121 kb on - strand, within Rv3605cat 4048.237 kb on + strandat 4048.769 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chlorhexidine diacetate salt 0.0021 mM
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4,047,596 + -0.4
4,047,872 - Rv3605c 0.35 -0.9
4,047,933 + Rv3605c 0.48 -0.7
4,048,018 + Rv3605c 0.66 -0.0
4,048,120 + Rv3605c 0.87 -0.7
4,048,120 + Rv3605c 0.87 -1.4
4,048,121 - Rv3605c 0.87 -1.5
4,048,237 + -0.0
4,048,769 - -0.0

Or see this region's nucleotide sequence