Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0090

Experiment: 7H9 with Chlorhexidine diacetate salt 0.0021 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0088 and Rv0089 are separated by 156 nucleotidesRv0089 and Rv0090 are separated by 128 nucleotidesRv0090 and Rv0091 are separated by 433 nucleotides Rv0088: Rv0088 - Possible polyketide cyclase/dehydrase, at 96,927 to 97,601 Rv0088 Rv0089: Rv0089 - Possible methyltransferase/methylase, at 97,758 to 98,351 Rv0089 Rv0090: Rv0090 - Possible membrane protein, at 98,480 to 99,250 Rv0090 Rv0091: Rv0091 - Probable bifunctional MTA/SAH nucleosidase Mtn: 5'-methylthioadenosine nucleosidase (methylthioadenosine methylthioribohydrolase) + S-adenosylhomocysteine nucleosidase (S-adenosyl-L-homocysteine homocysteinylribohydrolase), at 99,684 to 100,451 Rv0091 Position (kb) 98 99 100Strain fitness (log2 ratio) -2 -1 0 1 2at 97.581 kb on + strandat 97.668 kb on + strandat 97.707 kb on + strandat 97.707 kb on + strandat 97.888 kb on + strand, within Rv0089at 97.893 kb on + strand, within Rv0089at 98.261 kb on - strand, within Rv0089at 98.397 kb on - strandat 98.437 kb on + strandat 98.468 kb on + strandat 98.468 kb on + strandat 98.469 kb on - strandat 98.469 kb on - strandat 98.614 kb on - strand, within Rv0090at 98.693 kb on - strand, within Rv0090at 98.819 kb on - strand, within Rv0090at 99.137 kb on + strand, within Rv0090at 99.138 kb on - strand, within Rv0090at 99.188 kb on + strandat 99.940 kb on + strand, within Rv0091at 99.941 kb on - strand, within Rv0091at 99.941 kb on - strand, within Rv0091at 100.021 kb on - strand, within Rv0091at 100.176 kb on + strand, within Rv0091at 100.179 kb on + strand, within Rv0091at 100.179 kb on + strand, within Rv0091at 100.207 kb on - strand, within Rv0091at 100.207 kb on - strand, within Rv0091

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Chlorhexidine diacetate salt 0.0021 mM
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97,581 + +0.2
97,668 + -0.3
97,707 + +0.1
97,707 + +0.0
97,888 + Rv0089 0.22 -0.3
97,893 + Rv0089 0.23 +0.3
98,261 - Rv0089 0.85 -0.8
98,397 - +0.0
98,437 + -0.4
98,468 + -0.6
98,468 + +0.0
98,469 - +0.9
98,469 - -2.3
98,614 - Rv0090 0.17 +1.0
98,693 - Rv0090 0.28 -1.0
98,819 - Rv0090 0.44 -1.2
99,137 + Rv0090 0.85 -0.3
99,138 - Rv0090 0.85 +2.0
99,188 + +0.7
99,940 + Rv0091 0.33 +1.0
99,941 - Rv0091 0.33 -0.3
99,941 - Rv0091 0.33 -0.8
100,021 - Rv0091 0.44 +1.7
100,176 + Rv0091 0.64 -0.2
100,179 + Rv0091 0.64 +0.4
100,179 + Rv0091 0.64 +0.8
100,207 - Rv0091 0.68 +0.6
100,207 - Rv0091 0.68 -0.0

Or see this region's nucleotide sequence