Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1840c

Experiment: 7H9 with Tetrazolium violet 0.065 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1837c and Rv1838c are separated by 275 nucleotidesRv1838c and Rv1839c overlap by 4 nucleotidesRv1839c and Rv1840c are separated by 58 nucleotidesRv1840c and Rv1841c are separated by 162 nucleotides Rv1837c: Rv1837c - Malate synthase G GlcB, at 2,084,756 to 2,086,981 Rv1837c Rv1838c: Rv1838c - Possible toxin VapC13, at 2,087,257 to 2,087,652 Rv1838c Rv1839c: Rv1839c - Possible antitoxin VapB13, at 2,087,649 to 2,087,912 Rv1839c Rv1840c: Rv1840c - PE-PGRS family protein PE_PGRS34, at 2,087,971 to 2,089,518 Rv1840c Rv1841c: Rv1841c - Conserved hypothetical membrane protein, at 2,089,681 to 2,090,718 Rv1841c Position (kb) 2087 2088 2089 2090Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2086.994 kb on + strandat 2087.014 kb on - strandat 2087.032 kb on + strandat 2087.032 kb on + strandat 2087.032 kb on + strandat 2087.033 kb on - strandat 2087.033 kb on - strandat 2087.035 kb on + strandat 2087.035 kb on + strandat 2087.036 kb on - strandat 2087.139 kb on + strandat 2087.162 kb on + strandat 2087.163 kb on - strandat 2087.163 kb on - strandat 2087.283 kb on - strandat 2087.350 kb on + strand, within Rv1838cat 2087.350 kb on + strand, within Rv1838cat 2087.350 kb on + strand, within Rv1838cat 2087.350 kb on + strand, within Rv1838cat 2087.350 kb on + strand, within Rv1838cat 2087.351 kb on - strand, within Rv1838cat 2087.351 kb on - strand, within Rv1838cat 2087.351 kb on - strand, within Rv1838cat 2087.351 kb on - strand, within Rv1838cat 2087.351 kb on - strand, within Rv1838cat 2087.351 kb on - strand, within Rv1838cat 2087.451 kb on + strand, within Rv1838cat 2087.501 kb on + strand, within Rv1838cat 2087.501 kb on + strand, within Rv1838cat 2087.501 kb on + strand, within Rv1838cat 2087.512 kb on + strand, within Rv1838cat 2087.512 kb on + strand, within Rv1838cat 2087.512 kb on + strand, within Rv1838cat 2087.513 kb on - strand, within Rv1838cat 2087.513 kb on - strand, within Rv1838cat 2087.513 kb on - strand, within Rv1838cat 2087.513 kb on - strand, within Rv1838cat 2087.543 kb on - strand, within Rv1838cat 2087.595 kb on + strand, within Rv1838cat 2087.621 kb on + strandat 2087.622 kb on - strandat 2087.622 kb on - strandat 2087.622 kb on - strandat 2087.622 kb on - strandat 2087.622 kb on - strandat 2087.769 kb on + strand, within Rv1839cat 2087.770 kb on - strand, within Rv1839cat 2087.770 kb on - strand, within Rv1839cat 2087.770 kb on - strand, within Rv1839cat 2087.913 kb on - strandat 2087.926 kb on - strandat 2087.934 kb on + strandat 2087.935 kb on - strandat 2087.935 kb on - strandat 2087.973 kb on - strandat 2089.260 kb on - strand, within Rv1840cat 2089.260 kb on - strand, within Rv1840cat 2089.566 kb on + strandat 2089.566 kb on + strandat 2089.566 kb on + strandat 2089.567 kb on - strandat 2089.567 kb on - strandat 2089.643 kb on - strandat 2089.775 kb on - strandat 2089.928 kb on + strand, within Rv1841cat 2089.929 kb on - strand, within Rv1841cat 2090.239 kb on + strand, within Rv1841cat 2090.294 kb on + strand, within Rv1841cat 2090.295 kb on - strand, within Rv1841cat 2090.303 kb on + strand, within Rv1841cat 2090.304 kb on - strand, within Rv1841cat 2090.461 kb on + strand, within Rv1841cat 2090.462 kb on - strand, within Rv1841cat 2090.462 kb on - strand, within Rv1841cat 2090.462 kb on - strand, within Rv1841c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Tetrazolium violet 0.065 mM
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2,086,994 + -0.2
2,087,014 - +0.9
2,087,032 + -0.4
2,087,032 + -0.1
2,087,032 + -0.2
2,087,033 - -0.3
2,087,033 - -0.4
2,087,035 + -0.6
2,087,035 + -0.5
2,087,036 - +0.6
2,087,139 + -0.4
2,087,162 + -1.0
2,087,163 - -0.3
2,087,163 - -0.6
2,087,283 - -0.2
2,087,350 + Rv1838c 0.23 -0.5
2,087,350 + Rv1838c 0.23 -0.1
2,087,350 + Rv1838c 0.23 +0.2
2,087,350 + Rv1838c 0.23 +0.0
2,087,350 + Rv1838c 0.23 -0.4
2,087,351 - Rv1838c 0.24 -0.1
2,087,351 - Rv1838c 0.24 +0.1
2,087,351 - Rv1838c 0.24 -0.3
2,087,351 - Rv1838c 0.24 -0.9
2,087,351 - Rv1838c 0.24 -2.7
2,087,351 - Rv1838c 0.24 -1.5
2,087,451 + Rv1838c 0.49 -0.3
2,087,501 + Rv1838c 0.62 -0.8
2,087,501 + Rv1838c 0.62 +0.2
2,087,501 + Rv1838c 0.62 -0.3
2,087,512 + Rv1838c 0.64 -0.6
2,087,512 + Rv1838c 0.64 -0.1
2,087,512 + Rv1838c 0.64 -0.3
2,087,513 - Rv1838c 0.65 -0.6
2,087,513 - Rv1838c 0.65 +0.1
2,087,513 - Rv1838c 0.65 +1.8
2,087,513 - Rv1838c 0.65 -1.9
2,087,543 - Rv1838c 0.72 -0.4
2,087,595 + Rv1838c 0.85 +0.1
2,087,621 + +1.8
2,087,622 - -1.4
2,087,622 - -1.6
2,087,622 - -0.4
2,087,622 - -0.2
2,087,622 - -0.2
2,087,769 + Rv1839c 0.45 +0.2
2,087,770 - Rv1839c 0.46 -1.8
2,087,770 - Rv1839c 0.46 -0.6
2,087,770 - Rv1839c 0.46 +1.3
2,087,913 - +0.1
2,087,926 - +0.7
2,087,934 + -0.3
2,087,935 - -0.8
2,087,935 - -0.7
2,087,973 - +1.8
2,089,260 - Rv1840c 0.83 -0.7
2,089,260 - Rv1840c 0.83 -0.8
2,089,566 + +0.1
2,089,566 + -0.1
2,089,566 + -0.5
2,089,567 - -0.1
2,089,567 - +0.2
2,089,643 - +0.4
2,089,775 - +0.4
2,089,928 + Rv1841c 0.24 -0.8
2,089,929 - Rv1841c 0.24 -0.1
2,090,239 + Rv1841c 0.54 -0.3
2,090,294 + Rv1841c 0.59 +2.0
2,090,295 - Rv1841c 0.59 +3.8
2,090,303 + Rv1841c 0.60 -1.0
2,090,304 - Rv1841c 0.60 -0.3
2,090,461 + Rv1841c 0.75 +0.3
2,090,462 - Rv1841c 0.75 -0.4
2,090,462 - Rv1841c 0.75 +0.8
2,090,462 - Rv1841c 0.75 -0.3

Or see this region's nucleotide sequence