Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0219

Experiment: 7H9 with Tetrazolium violet 0.065 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0218 and Rv0219 are separated by 1 nucleotidesRv0219 and Rv0220 are separated by 9 nucleotides Rv0218: Rv0218 - Probable conserved transmembrane protein, at 260,924 to 262,252 Rv0218 Rv0219: Rv0219 - Probable conserved transmembrane protein, at 262,254 to 262,802 Rv0219 Rv0220: Rv0220 - Probable esterase LipC, at 262,812 to 264,023 Rv0220 Position (kb) 262 263Strain fitness (log2 ratio) -2 -1 0 1 2at 261.431 kb on + strand, within Rv0218at 261.432 kb on - strand, within Rv0218at 261.438 kb on - strand, within Rv0218at 261.511 kb on + strand, within Rv0218at 261.888 kb on + strand, within Rv0218at 262.048 kb on + strand, within Rv0218at 262.344 kb on + strand, within Rv0219at 262.551 kb on + strand, within Rv0219at 262.632 kb on + strand, within Rv0219at 262.632 kb on + strand, within Rv0219at 262.632 kb on + strand, within Rv0219at 262.723 kb on - strand, within Rv0219at 262.868 kb on - strandat 262.889 kb on + strandat 262.890 kb on - strandat 263.246 kb on - strand, within Rv0220at 263.262 kb on - strand, within Rv0220at 263.631 kb on + strand, within Rv0220at 263.640 kb on + strand, within Rv0220

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Tetrazolium violet 0.065 mM
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261,431 + Rv0218 0.38 +0.8
261,432 - Rv0218 0.38 +0.1
261,438 - Rv0218 0.39 -0.1
261,511 + Rv0218 0.44 -0.3
261,888 + Rv0218 0.73 +1.8
262,048 + Rv0218 0.85 -2.1
262,344 + Rv0219 0.16 -1.7
262,551 + Rv0219 0.54 -0.9
262,632 + Rv0219 0.69 -0.6
262,632 + Rv0219 0.69 +0.0
262,632 + Rv0219 0.69 -0.9
262,723 - Rv0219 0.85 -1.3
262,868 - -0.7
262,889 + -0.4
262,890 - -0.3
263,246 - Rv0220 0.36 +0.2
263,262 - Rv0220 0.37 -0.6
263,631 + Rv0220 0.68 -0.8
263,640 + Rv0220 0.68 +1.8

Or see this region's nucleotide sequence