Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2553c

Experiment: 7H9 with Tetrazolium violet 0.065 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2551c and Rv2552c are separated by 11 nucleotidesRv2552c and Rv2553c overlap by 4 nucleotidesRv2553c and Rv2554c overlap by 8 nucleotidesRv2554c and Rv2555c are separated by 0 nucleotides Rv2551c: Rv2551c - Conserved hypothetical protein, at 2,870,775 to 2,871,194 Rv2551c Rv2552c: Rv2552c - Probable shikimate 5-dehydrogenase AroE (5-dehydroshikimate reductase), at 2,871,206 to 2,872,015 Rv2552c Rv2553c: Rv2553c - Probable conserved membrane protein, at 2,872,012 to 2,873,265 Rv2553c Rv2554c: Rv2554c - Conserved protein, at 2,873,258 to 2,873,770 Rv2554c Rv2555c: Rv2555c - Probable alanyl-tRNA synthetase AlaS (alanine--tRNA ligase) (alanine translase) (ALARS), at 2,873,771 to 2,876,485 Rv2555c Position (kb) 2872 2873 2874Strain fitness (log2 ratio) -1 0 1at 2871.019 kb on + strand, within Rv2551cat 2871.020 kb on - strand, within Rv2551cat 2871.208 kb on - strandat 2871.208 kb on - strandat 2872.125 kb on - strandat 2872.416 kb on - strand, within Rv2553cat 2872.875 kb on - strand, within Rv2553c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Tetrazolium violet 0.065 mM
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2,871,019 + Rv2551c 0.58 +0.1
2,871,020 - Rv2551c 0.58 -0.4
2,871,208 - +0.6
2,871,208 - +0.0
2,872,125 - -0.4
2,872,416 - Rv2553c 0.32 -1.5
2,872,875 - Rv2553c 0.69 -0.3

Or see this region's nucleotide sequence