Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0623

Experiment: 7H9 with Tetrazolium violet 0.065 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0622 and Rv0623 are separated by 92 nucleotidesRv0623 and Rv0624 overlap by 1 nucleotidesRv0624 and Rv0625c are separated by 93 nucleotides Rv0622: Rv0622 - Possible membrane protein, at 715,370 to 716,317 Rv0622 Rv0623: Rv0623 - Possible antitoxin VapB30, at 716,410 to 716,664 Rv0623 Rv0624: Rv0624 - Possible toxin VapC30. Contains PIN domain., at 716,664 to 717,059 Rv0624 Rv0625c: Rv0625c - Probable conserved transmembrane protein, at 717,153 to 717,893 Rv0625c Position (kb) 716 717Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 715.437 kb on - strandat 715.542 kb on - strand, within Rv0622at 715.542 kb on - strand, within Rv0622at 715.796 kb on + strand, within Rv0622at 715.797 kb on - strand, within Rv0622at 716.231 kb on + strandat 716.232 kb on - strandat 716.422 kb on - strandat 716.422 kb on - strandat 716.638 kb on + strand, within Rv0623at 716.639 kb on - strandat 716.797 kb on + strand, within Rv0624at 716.798 kb on - strand, within Rv0624at 716.919 kb on + strand, within Rv0624at 716.920 kb on - strand, within Rv0624at 716.952 kb on + strand, within Rv0624at 716.953 kb on - strand, within Rv0624at 716.970 kb on + strand, within Rv0624at 716.971 kb on - strand, within Rv0624at 717.057 kb on + strandat 717.058 kb on - strandat 717.058 kb on - strandat 717.064 kb on + strandat 717.428 kb on - strand, within Rv0625cat 717.428 kb on - strand, within Rv0625c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Tetrazolium violet 0.065 mM
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715,437 - -1.0
715,542 - Rv0622 0.18 -1.7
715,542 - Rv0622 0.18 +1.8
715,796 + Rv0622 0.45 -0.0
715,797 - Rv0622 0.45 -1.1
716,231 + -0.7
716,232 - -0.8
716,422 - +0.0
716,422 - -0.4
716,638 + Rv0623 0.89 -0.5
716,639 - -1.5
716,797 + Rv0624 0.34 -0.8
716,798 - Rv0624 0.34 -0.6
716,919 + Rv0624 0.64 +0.1
716,920 - Rv0624 0.65 +0.2
716,952 + Rv0624 0.73 -0.9
716,953 - Rv0624 0.73 +2.8
716,970 + Rv0624 0.77 -0.6
716,971 - Rv0624 0.78 -0.9
717,057 + +0.3
717,058 - -3.5
717,058 - -1.6
717,064 + +0.4
717,428 - Rv0625c 0.37 -0.3
717,428 - Rv0625c 0.37 +0.4

Or see this region's nucleotide sequence