Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3149

Experiment: 7H9 with Dibucaine 0.21 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3148 and Rv3149 overlap by 4 nucleotidesRv3149 and Rv3150 overlap by 4 nucleotides Rv3148: Rv3148 - Probable NADH dehydrogenase I (chain D) NuoD (NADH-ubiquinone oxidoreductase chain D), at 3,513,338 to 3,514,660 Rv3148 Rv3149: Rv3149 - Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E), at 3,514,657 to 3,515,415 Rv3149 Rv3150: Rv3150 - Probable NADH dehydrogenase I (chain F) NuoF (NADH-ubiquinone oxidoreductase chain F), at 3,515,412 to 3,516,749 Rv3150 Position (kb) 3514 3515 3516Strain fitness (log2 ratio) -1 0 1at 3513.897 kb on - strand, within Rv3148at 3514.160 kb on + strand, within Rv3148at 3514.273 kb on - strand, within Rv3148at 3514.273 kb on - strand, within Rv3148at 3514.494 kb on + strand, within Rv3148at 3514.495 kb on - strand, within Rv3148at 3514.539 kb on - strandat 3514.763 kb on + strand, within Rv3149at 3514.868 kb on - strand, within Rv3149at 3514.948 kb on + strand, within Rv3149at 3514.948 kb on + strand, within Rv3149at 3514.949 kb on - strand, within Rv3149at 3514.957 kb on + strand, within Rv3149at 3514.957 kb on + strand, within Rv3149at 3514.958 kb on - strand, within Rv3149at 3514.958 kb on - strand, within Rv3149at 3515.297 kb on + strand, within Rv3149at 3515.491 kb on - strandat 3515.871 kb on + strand, within Rv3150at 3515.952 kb on + strand, within Rv3150at 3516.168 kb on + strand, within Rv3150at 3516.168 kb on + strand, within Rv3150at 3516.224 kb on + strand, within Rv3150

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Dibucaine 0.21 mM
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3,513,897 - Rv3148 0.42 -0.2
3,514,160 + Rv3148 0.62 -1.0
3,514,273 - Rv3148 0.71 -1.1
3,514,273 - Rv3148 0.71 +0.9
3,514,494 + Rv3148 0.87 -0.7
3,514,495 - Rv3148 0.87 -0.5
3,514,539 - -0.5
3,514,763 + Rv3149 0.14 -1.2
3,514,868 - Rv3149 0.28 -1.2
3,514,948 + Rv3149 0.38 -0.5
3,514,948 + Rv3149 0.38 -0.6
3,514,949 - Rv3149 0.38 -0.8
3,514,957 + Rv3149 0.40 -0.7
3,514,957 + Rv3149 0.40 -0.7
3,514,958 - Rv3149 0.40 -0.4
3,514,958 - Rv3149 0.40 -0.2
3,515,297 + Rv3149 0.84 -1.2
3,515,491 - +0.1
3,515,871 + Rv3150 0.34 -0.4
3,515,952 + Rv3150 0.40 -1.2
3,516,168 + Rv3150 0.57 -0.5
3,516,168 + Rv3150 0.57 -0.2
3,516,224 + Rv3150 0.61 +0.3

Or see this region's nucleotide sequence