Experiment: 7H9 with Dibucaine 0.21 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0458 and Rv0459 overlap by 1 nucleotides Rv0459 and Rv0460 are separated by 59 nucleotides Rv0460 and Rv0461 are separated by 37 nucleotides Rv0461 and Rv0462 are separated by 63 nucleotides
Rv0458: Rv0458 - Probable aldehyde dehydrogenase, at 549,675 to 551,198
Rv0458
Rv0459: Rv0459 - Conserved hypothetical protein, at 551,198 to 551,689
Rv0459
Rv0460: Rv0460 - Conserved hydrophobic protein, at 551,749 to 551,988
Rv0460
Rv0461: Rv0461 - Probable transmembrane protein, at 552,026 to 552,550
Rv0461
Rv0462: Rv0462 - Dihydrolipoamide dehydrogenase LpdC (lipoamide reductase (NADH)) (lipoyl dehydrogenase) (dihydrolipoyl dehydrogenase) (diaphorase), at 552,614 to 554,008
Rv0462
Position (kb)
551
552 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 550.824 kb on + strand, within Rv0458 at 550.827 kb on + strand, within Rv0458 at 550.827 kb on + strand, within Rv0458 at 550.827 kb on + strand, within Rv0458 at 550.828 kb on - strand, within Rv0458 at 550.828 kb on - strand, within Rv0458 at 550.828 kb on - strand, within Rv0458 at 550.828 kb on - strand, within Rv0458 at 550.923 kb on + strand, within Rv0458 at 551.053 kb on - strand at 551.088 kb on + strand at 551.089 kb on - strand at 551.139 kb on + strand at 551.139 kb on + strand at 551.256 kb on + strand, within Rv0459 at 551.257 kb on - strand, within Rv0459 at 551.426 kb on + strand, within Rv0459 at 551.426 kb on + strand, within Rv0459 at 551.594 kb on + strand, within Rv0459 at 551.616 kb on + strand, within Rv0459 at 551.617 kb on - strand, within Rv0459 at 551.696 kb on + strand at 551.697 kb on - strand at 551.704 kb on + strand at 551.705 kb on - strand at 551.844 kb on - strand, within Rv0460 at 551.844 kb on - strand, within Rv0460 at 552.217 kb on - strand, within Rv0461 at 552.228 kb on - strand, within Rv0461 at 552.230 kb on - strand, within Rv0461 at 552.230 kb on - strand, within Rv0461 at 552.230 kb on - strand, within Rv0461 at 552.234 kb on - strand, within Rv0461 at 552.234 kb on - strand, within Rv0461 at 552.234 kb on - strand, within Rv0461 at 552.258 kb on + strand, within Rv0461 at 552.273 kb on + strand, within Rv0461 at 552.273 kb on + strand, within Rv0461 at 552.320 kb on + strand, within Rv0461 at 552.365 kb on + strand, within Rv0461 at 552.366 kb on - strand, within Rv0461 at 552.492 kb on - strand, within Rv0461 at 552.500 kb on + strand at 552.500 kb on + strand at 552.500 kb on + strand at 552.500 kb on + strand at 552.500 kb on + strand at 552.501 kb on - strand at 552.501 kb on - strand at 552.545 kb on + strand at 552.546 kb on - strand at 552.546 kb on - strand at 552.548 kb on + strand at 552.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dibucaine 0.21 mM remove 550,824 + Rv0458 0.75 -0.2 550,827 + Rv0458 0.76 +0.3 550,827 + Rv0458 0.76 +0.1 550,827 + Rv0458 0.76 -0.6 550,828 - Rv0458 0.76 -2.4 550,828 - Rv0458 0.76 +0.1 550,828 - Rv0458 0.76 -0.6 550,828 - Rv0458 0.76 +0.4 550,923 + Rv0458 0.82 -1.2 551,053 - -0.3 551,088 + +0.2 551,089 - +0.8 551,139 + +0.8 551,139 + -0.7 551,256 + Rv0459 0.12 -0.3 551,257 - Rv0459 0.12 +0.7 551,426 + Rv0459 0.46 -1.8 551,426 + Rv0459 0.46 -0.1 551,594 + Rv0459 0.80 +0.2 551,616 + Rv0459 0.85 -0.5 551,617 - Rv0459 0.85 -0.4 551,696 + +0.1 551,697 - -1.3 551,704 + -1.7 551,705 - +0.1 551,844 - Rv0460 0.40 -0.6 551,844 - Rv0460 0.40 -0.9 552,217 - Rv0461 0.36 +1.9 552,228 - Rv0461 0.38 -0.6 552,230 - Rv0461 0.39 -0.7 552,230 - Rv0461 0.39 -1.3 552,230 - Rv0461 0.39 -0.2 552,234 - Rv0461 0.40 +0.3 552,234 - Rv0461 0.40 +0.2 552,234 - Rv0461 0.40 -0.4 552,258 + Rv0461 0.44 +0.4 552,273 + Rv0461 0.47 -0.3 552,273 + Rv0461 0.47 -0.5 552,320 + Rv0461 0.56 +0.0 552,365 + Rv0461 0.65 -0.9 552,366 - Rv0461 0.65 -0.0 552,492 - Rv0461 0.89 -0.1 552,500 + +1.9 552,500 + +0.6 552,500 + -1.3 552,500 + -0.7 552,500 + -0.7 552,501 - -0.6 552,501 - -0.2 552,545 + +0.4 552,546 - -0.2 552,546 - +1.3 552,548 + -0.0 552,570 + +0.9
Or see this region's nucleotide sequence