Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0735

Experiment: 7H9 with Phenazine methosulfate 0.0054 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0733 and Rv0734 are separated by 2 nucleotidesRv0734 and Rv0735 are separated by 72 nucleotidesRv0735 and Rv0736 are separated by 63 nucleotides Rv0733: Rv0733 - Adenylate kinase Adk (ATP-AMP transphosphorylase), at 826,122 to 826,667 Rv0733 Rv0734: Rv0734 - Methionine aminopeptidase MapA (map) (peptidase M) (MetAP), at 826,670 to 827,470 Rv0734 Rv0735: Rv0735 - Probable alternative RNA polymerase sigma factor SigL, at 827,543 to 828,076 Rv0735 Rv0736: Rv0736 - Anti-sigma factor RslA, at 828,140 to 828,892 Rv0736 Position (kb) 827 828 829Strain fitness (log2 ratio) -1 0 1 2at 826.665 kb on + strandat 826.978 kb on - strand, within Rv0734at 827.248 kb on + strand, within Rv0734at 827.249 kb on - strand, within Rv0734at 827.468 kb on + strandat 827.598 kb on - strand, within Rv0735at 827.628 kb on - strand, within Rv0735at 827.923 kb on + strand, within Rv0735at 827.924 kb on - strand, within Rv0735at 827.946 kb on - strand, within Rv0735at 827.948 kb on + strand, within Rv0735at 828.023 kb on + strandat 828.024 kb on - strandat 828.067 kb on + strandat 828.112 kb on + strandat 828.112 kb on + strandat 828.890 kb on + strandat 828.890 kb on + strandat 828.903 kb on + strandat 828.904 kb on - strandat 828.922 kb on + strandat 828.922 kb on + strandat 828.935 kb on + strandat 828.936 kb on - strandat 828.936 kb on - strandat 828.936 kb on - strandat 829.065 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Phenazine methosulfate 0.0054 mM
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826,665 + -0.6
826,978 - Rv0734 0.38 -1.1
827,248 + Rv0734 0.72 +1.8
827,249 - Rv0734 0.72 -0.6
827,468 + +1.8
827,598 - Rv0735 0.10 -1.3
827,628 - Rv0735 0.16 -1.0
827,923 + Rv0735 0.71 -0.6
827,924 - Rv0735 0.71 -0.2
827,946 - Rv0735 0.75 -0.7
827,948 + Rv0735 0.76 +0.2
828,023 + +0.8
828,024 - -0.3
828,067 + -0.5
828,112 + -0.4
828,112 + -0.6
828,890 + -0.2
828,890 + -0.2
828,903 + -1.2
828,904 - +0.7
828,922 + -1.3
828,922 + -0.6
828,935 + +0.2
828,936 - -1.0
828,936 - -0.3
828,936 - -0.2
829,065 - +0.5

Or see this region's nucleotide sequence