Experiment: 7H9 with Phenazine methosulfate 0.0054 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0454 and Rv0455c are separated by 189 nucleotides Rv0455c and Rv0456c are separated by 67 nucleotides Rv0456c and Rv0456A are separated by 272 nucleotides Rv0456A and Rv0456B overlap by 14 nucleotides Rv0456B and Rv0457c are separated by 68 nucleotides
Rv0454: Rv0454 - Conserved hypothetical protein, at 544,835 to 545,185
Rv0454
Rv0455c: Rv0455c - Conserved protein, at 545,375 to 545,821
Rv0455c
Rv0456c: Rv0456c - enoyl-CoA hydratase EchA2 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 545,889 to 546,803
Rv0456c
Rv0456A: Rv0456A - Possible toxin MazF1, at 547,076 to 547,357
Rv0456A
Rv0456B: Rv0456B - Possible antitoxin MazE1, at 547,344 to 547,517
Rv0456B
Rv0457c: Rv0457c - Probable peptidase, at 547,586 to 549,607
Rv0457c
Position (kb) 545
546
547 Strain fitness (log2 ratio) -2
-1
0
1
2 at 544.924 kb on + strand, within Rv0454 at 544.924 kb on + strand, within Rv0454 at 544.925 kb on - strand, within Rv0454 at 544.925 kb on - strand, within Rv0454 at 544.935 kb on - strand, within Rv0454 at 544.935 kb on - strand, within Rv0454 at 544.956 kb on + strand, within Rv0454 at 544.956 kb on + strand, within Rv0454 at 544.957 kb on - strand, within Rv0454 at 544.957 kb on - strand, within Rv0454 at 545.101 kb on + strand, within Rv0454 at 545.102 kb on - strand, within Rv0454 at 545.102 kb on - strand, within Rv0454 at 545.102 kb on - strand, within Rv0454 at 545.126 kb on - strand, within Rv0454 at 545.183 kb on + strand at 545.190 kb on - strand at 545.377 kb on - strand at 545.822 kb on - strand at 545.834 kb on - strand at 545.890 kb on + strand at 545.956 kb on + strand at 546.109 kb on + strand, within Rv0456c at 546.109 kb on + strand, within Rv0456c at 546.311 kb on - strand, within Rv0456c at 546.331 kb on + strand, within Rv0456c at 546.331 kb on + strand, within Rv0456c at 546.331 kb on + strand, within Rv0456c at 546.352 kb on + strand, within Rv0456c at 546.352 kb on + strand, within Rv0456c at 546.601 kb on + strand, within Rv0456c at 546.772 kb on + strand at 546.772 kb on + strand at 546.772 kb on + strand at 546.773 kb on - strand at 546.803 kb on + strand at 546.804 kb on - strand at 547.036 kb on + strand at 547.036 kb on + strand at 547.036 kb on + strand at 547.037 kb on - strand at 547.245 kb on - strand, within Rv0456A at 547.360 kb on - strand at 547.474 kb on - strand, within Rv0456B at 547.481 kb on + strand, within Rv0456B at 547.481 kb on + strand, within Rv0456B at 547.482 kb on - strand, within Rv0456B at 547.482 kb on - strand, within Rv0456B at 547.482 kb on - strand, within Rv0456B at 547.482 kb on - strand, within Rv0456B at 547.484 kb on + strand, within Rv0456B at 547.491 kb on + strand, within Rv0456B at 547.492 kb on - strand, within Rv0456B at 547.494 kb on + strand, within Rv0456B at 547.497 kb on + strand, within Rv0456B at 547.497 kb on + strand, within Rv0456B at 547.505 kb on - strand at 547.507 kb on + strand at 547.515 kb on - strand at 547.690 kb on - strand at 547.787 kb on + strand at 547.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Phenazine methosulfate 0.0054 mM remove 544,924 + Rv0454 0.25 -0.5 544,924 + Rv0454 0.25 +0.2 544,925 - Rv0454 0.26 -0.3 544,925 - Rv0454 0.26 +0.1 544,935 - Rv0454 0.28 -0.5 544,935 - Rv0454 0.28 +2.4 544,956 + Rv0454 0.34 +0.8 544,956 + Rv0454 0.34 -0.0 544,957 - Rv0454 0.35 +1.0 544,957 - Rv0454 0.35 +0.1 545,101 + Rv0454 0.76 -0.8 545,102 - Rv0454 0.76 +0.0 545,102 - Rv0454 0.76 -0.4 545,102 - Rv0454 0.76 -0.8 545,126 - Rv0454 0.83 -1.3 545,183 + +0.0 545,190 - -0.2 545,377 - +2.0 545,822 - -1.2 545,834 - -0.2 545,890 + -0.0 545,956 + -1.6 546,109 + Rv0456c 0.24 -2.3 546,109 + Rv0456c 0.24 +0.1 546,311 - Rv0456c 0.46 +2.5 546,331 + Rv0456c 0.48 +0.9 546,331 + Rv0456c 0.48 -0.2 546,331 + Rv0456c 0.48 +0.6 546,352 + Rv0456c 0.51 -0.1 546,352 + Rv0456c 0.51 +0.7 546,601 + Rv0456c 0.78 +1.1 546,772 + +0.3 546,772 + +0.8 546,772 + -0.9 546,773 - -0.0 546,803 + +1.4 546,804 - -0.8 547,036 + -0.1 547,036 + -0.0 547,036 + -0.3 547,037 - +1.0 547,245 - Rv0456A 0.60 +1.3 547,360 - -1.2 547,474 - Rv0456B 0.75 +0.1 547,481 + Rv0456B 0.79 -1.2 547,481 + Rv0456B 0.79 +0.6 547,482 - Rv0456B 0.79 -1.0 547,482 - Rv0456B 0.79 -0.2 547,482 - Rv0456B 0.79 -0.7 547,482 - Rv0456B 0.79 -0.6 547,484 + Rv0456B 0.80 +0.9 547,491 + Rv0456B 0.84 -0.4 547,492 - Rv0456B 0.85 +0.1 547,494 + Rv0456B 0.86 +0.2 547,497 + Rv0456B 0.88 -1.0 547,497 + Rv0456B 0.88 -0.2 547,505 - -1.1 547,507 + +0.8 547,515 - -1.5 547,690 - +0.7 547,787 + -0.5 547,787 + +0.6
Or see this region's nucleotide sequence