Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3331

Experiment: 7H9 with Tetracycline 0.0058 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3330 and Rv3331 are separated by 95 nucleotidesRv3331 and Rv3332 overlap by 4 nucleotides Rv3330: Rv3330 - Probable penicillin-binding protein DacB1 (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP) (DD-transpeptidase) (serine-type D-ala-D-ala carboxypeptidase) (D-amino acid hydrolase), at 3,715,777 to 3,716,994 Rv3330 Rv3331: Rv3331 - Probable sugar-transport integral membrane protein SugI, at 3,717,090 to 3,718,598 Rv3331 Rv3332: Rv3332 - Probable N-acetylglucosamine-6-phosphate deacetylase NagA (GlcNAc 6-P deacetylase), at 3,718,595 to 3,719,746 Rv3332 Position (kb) 3717 3718 3719Strain fitness (log2 ratio) -1 0 1at 3716.263 kb on + strand, within Rv3330at 3716.263 kb on + strand, within Rv3330at 3716.264 kb on - strand, within Rv3330at 3716.264 kb on - strand, within Rv3330at 3716.264 kb on - strand, within Rv3330at 3716.264 kb on - strand, within Rv3330at 3716.264 kb on - strand, within Rv3330at 3716.458 kb on + strand, within Rv3330at 3716.458 kb on + strand, within Rv3330at 3716.594 kb on - strand, within Rv3330at 3716.594 kb on - strand, within Rv3330at 3716.600 kb on - strand, within Rv3330at 3716.630 kb on - strand, within Rv3330at 3716.631 kb on + strand, within Rv3330at 3716.632 kb on - strand, within Rv3330at 3716.632 kb on - strand, within Rv3330at 3716.759 kb on - strand, within Rv3330at 3716.992 kb on + strandat 3716.993 kb on - strandat 3716.993 kb on - strandat 3717.000 kb on + strandat 3717.288 kb on + strand, within Rv3331at 3717.582 kb on + strand, within Rv3331at 3717.582 kb on + strand, within Rv3331at 3717.583 kb on - strand, within Rv3331at 3717.681 kb on + strand, within Rv3331at 3718.008 kb on + strand, within Rv3331at 3718.008 kb on + strand, within Rv3331at 3718.009 kb on - strand, within Rv3331at 3718.308 kb on + strand, within Rv3331at 3718.759 kb on + strand, within Rv3332at 3718.855 kb on + strand, within Rv3332

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Tetracycline 0.0058 mM
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3,716,263 + Rv3330 0.40 +1.6
3,716,263 + Rv3330 0.40 -0.4
3,716,264 - Rv3330 0.40 -1.0
3,716,264 - Rv3330 0.40 -0.5
3,716,264 - Rv3330 0.40 -0.4
3,716,264 - Rv3330 0.40 +0.1
3,716,264 - Rv3330 0.40 +0.2
3,716,458 + Rv3330 0.56 +1.0
3,716,458 + Rv3330 0.56 -0.6
3,716,594 - Rv3330 0.67 +0.1
3,716,594 - Rv3330 0.67 +0.8
3,716,600 - Rv3330 0.68 -1.3
3,716,630 - Rv3330 0.70 -0.2
3,716,631 + Rv3330 0.70 -0.3
3,716,632 - Rv3330 0.70 -0.6
3,716,632 - Rv3330 0.70 -0.6
3,716,759 - Rv3330 0.81 -1.4
3,716,992 + -1.1
3,716,993 - +0.4
3,716,993 - -1.4
3,717,000 + -0.4
3,717,288 + Rv3331 0.13 +0.4
3,717,582 + Rv3331 0.33 +1.5
3,717,582 + Rv3331 0.33 +0.3
3,717,583 - Rv3331 0.33 +1.5
3,717,681 + Rv3331 0.39 +0.2
3,718,008 + Rv3331 0.61 +1.1
3,718,008 + Rv3331 0.61 +0.7
3,718,009 - Rv3331 0.61 -0.4
3,718,308 + Rv3331 0.81 +0.7
3,718,759 + Rv3332 0.14 +0.2
3,718,855 + Rv3332 0.23 -0.2

Or see this region's nucleotide sequence