Experiment: 7H9 with 8-hydroxy-nitroquinoline 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0505c and Rv0506 are separated by 173 nucleotides Rv0506 and Rv0507 overlap by 4 nucleotides Rv0507 and Rv0508 overlap by 8 nucleotides Rv0508 and Rv0509 are separated by 49 nucleotides
Rv0505c: Rv0505c - Possible phosphoserine phosphatase SerB1 (PSP) (O-phosphoserine phosphohydrolase) (pspase), at 595,464 to 596,585
Rv0505c
Rv0506: Rv0506 - Probable conserved membrane protein MmpS2, at 596,759 to 597,202
Rv0506
Rv0507: Rv0507 - Probable conserved transmembrane transport protein MmpL2, at 597,199 to 600,105
Rv0507
Rv0508: Rv0508 - Conserved hypothetical protein, at 600,098 to 600,391
Rv0508
Rv0509: Rv0509 - Probable glutamyl-tRNA reductase HemA (GLUTR), at 600,441 to 601,847
Rv0509
Position (kb) 597
598
599
600
601 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 596.650 kb on - strand at 596.654 kb on + strand at 596.659 kb on - strand at 596.682 kb on + strand at 596.683 kb on - strand at 596.683 kb on - strand at 596.691 kb on + strand at 596.692 kb on - strand at 596.708 kb on + strand at 596.720 kb on + strand at 596.721 kb on - strand at 596.730 kb on + strand at 596.731 kb on - strand at 596.731 kb on - strand at 596.845 kb on + strand, within Rv0506 at 596.846 kb on - strand, within Rv0506 at 596.846 kb on - strand, within Rv0506 at 596.846 kb on - strand, within Rv0506 at 596.846 kb on - strand, within Rv0506 at 596.846 kb on - strand, within Rv0506 at 596.945 kb on + strand, within Rv0506 at 596.945 kb on + strand, within Rv0506 at 596.946 kb on - strand, within Rv0506 at 596.946 kb on - strand, within Rv0506 at 596.946 kb on - strand, within Rv0506 at 596.946 kb on - strand, within Rv0506 at 596.946 kb on - strand, within Rv0506 at 596.985 kb on - strand, within Rv0506 at 597.357 kb on - strand at 597.444 kb on - strand at 597.496 kb on + strand, within Rv0507 at 597.496 kb on + strand, within Rv0507 at 597.497 kb on - strand, within Rv0507 at 597.497 kb on - strand, within Rv0507 at 597.497 kb on - strand, within Rv0507 at 597.497 kb on - strand, within Rv0507 at 597.498 kb on + strand, within Rv0507 at 597.613 kb on + strand, within Rv0507 at 597.614 kb on - strand, within Rv0507 at 597.625 kb on + strand, within Rv0507 at 597.625 kb on + strand, within Rv0507 at 597.626 kb on - strand, within Rv0507 at 597.655 kb on + strand, within Rv0507 at 597.721 kb on + strand, within Rv0507 at 597.721 kb on + strand, within Rv0507 at 597.777 kb on + strand, within Rv0507 at 597.778 kb on - strand, within Rv0507 at 597.778 kb on - strand, within Rv0507 at 597.778 kb on - strand, within Rv0507 at 597.778 kb on - strand, within Rv0507 at 597.783 kb on + strand, within Rv0507 at 597.828 kb on - strand, within Rv0507 at 597.830 kb on - strand, within Rv0507 at 597.997 kb on + strand, within Rv0507 at 598.005 kb on - strand, within Rv0507 at 598.132 kb on + strand, within Rv0507 at 598.132 kb on + strand, within Rv0507 at 598.133 kb on - strand, within Rv0507 at 598.408 kb on + strand, within Rv0507 at 598.408 kb on + strand, within Rv0507 at 598.409 kb on - strand, within Rv0507 at 598.409 kb on - strand, within Rv0507 at 598.731 kb on - strand, within Rv0507 at 598.783 kb on + strand, within Rv0507 at 599.124 kb on + strand, within Rv0507 at 599.125 kb on - strand, within Rv0507 at 599.392 kb on - strand, within Rv0507 at 599.403 kb on - strand, within Rv0507 at 599.434 kb on + strand, within Rv0507 at 599.434 kb on + strand, within Rv0507 at 599.776 kb on - strand, within Rv0507 at 599.871 kb on + strand at 599.872 kb on - strand at 599.872 kb on - strand at 599.887 kb on - strand at 600.103 kb on + strand at 600.103 kb on + strand at 600.104 kb on - strand at 600.104 kb on - strand at 600.308 kb on + strand, within Rv0508 at 600.308 kb on + strand, within Rv0508 at 600.309 kb on - strand, within Rv0508 at 600.309 kb on - strand, within Rv0508 at 600.346 kb on - strand, within Rv0508 at 600.376 kb on + strand at 600.384 kb on + strand at 600.402 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with 8-hydroxy-nitroquinoline 0.03 mM remove 596,650 - -0.4 596,654 + -1.9 596,659 - -0.4 596,682 + -1.6 596,683 - +0.6 596,683 - -0.3 596,691 + -0.4 596,692 - +0.2 596,708 + -0.5 596,720 + -1.5 596,721 - +0.1 596,730 + -1.6 596,731 - +0.9 596,731 - +0.5 596,845 + Rv0506 0.19 +1.7 596,846 - Rv0506 0.20 -0.2 596,846 - Rv0506 0.20 +0.7 596,846 - Rv0506 0.20 -0.4 596,846 - Rv0506 0.20 -2.7 596,846 - Rv0506 0.20 +1.3 596,945 + Rv0506 0.42 -0.6 596,945 + Rv0506 0.42 -1.1 596,946 - Rv0506 0.42 -0.2 596,946 - Rv0506 0.42 -0.4 596,946 - Rv0506 0.42 -0.2 596,946 - Rv0506 0.42 +0.2 596,946 - Rv0506 0.42 -0.2 596,985 - Rv0506 0.51 -1.2 597,357 - -0.6 597,444 - +0.2 597,496 + Rv0507 0.10 +0.1 597,496 + Rv0507 0.10 -0.7 597,497 - Rv0507 0.10 +0.1 597,497 - Rv0507 0.10 -0.8 597,497 - Rv0507 0.10 +0.6 597,497 - Rv0507 0.10 +0.1 597,498 + Rv0507 0.10 -0.6 597,613 + Rv0507 0.14 -0.3 597,614 - Rv0507 0.14 -0.6 597,625 + Rv0507 0.15 +0.7 597,625 + Rv0507 0.15 -0.3 597,626 - Rv0507 0.15 -0.4 597,655 + Rv0507 0.16 +0.0 597,721 + Rv0507 0.18 -0.5 597,721 + Rv0507 0.18 +0.1 597,777 + Rv0507 0.20 -0.3 597,778 - Rv0507 0.20 +0.3 597,778 - Rv0507 0.20 -0.2 597,778 - Rv0507 0.20 +0.3 597,778 - Rv0507 0.20 -0.2 597,783 + Rv0507 0.20 +0.0 597,828 - Rv0507 0.22 -0.2 597,830 - Rv0507 0.22 -0.7 597,997 + Rv0507 0.27 -0.2 598,005 - Rv0507 0.28 -0.4 598,132 + Rv0507 0.32 +4.5 598,132 + Rv0507 0.32 -1.0 598,133 - Rv0507 0.32 -0.5 598,408 + Rv0507 0.42 +0.0 598,408 + Rv0507 0.42 +1.0 598,409 - Rv0507 0.42 +0.0 598,409 - Rv0507 0.42 -0.1 598,731 - Rv0507 0.53 +0.7 598,783 + Rv0507 0.54 -1.3 599,124 + Rv0507 0.66 +0.7 599,125 - Rv0507 0.66 +0.5 599,392 - Rv0507 0.75 +1.2 599,403 - Rv0507 0.76 -0.2 599,434 + Rv0507 0.77 +0.5 599,434 + Rv0507 0.77 +0.5 599,776 - Rv0507 0.89 -0.1 599,871 + -0.0 599,872 - -0.1 599,872 - -0.3 599,887 - -0.3 600,103 + -1.1 600,103 + -1.1 600,104 - -2.6 600,104 - -0.6 600,308 + Rv0508 0.71 -0.4 600,308 + Rv0508 0.71 +0.1 600,309 - Rv0508 0.72 -1.0 600,309 - Rv0508 0.72 -1.0 600,346 - Rv0508 0.84 -0.5 600,376 + -0.3 600,384 + -0.2 600,402 + -0.7
Or see this region's nucleotide sequence