Experiment: 7H9 with 8-hydroxy-nitroquinoline 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1415 and Rv1416 overlap by 4 nucleotides Rv1416 and Rv1417 overlap by 4 nucleotides Rv1417 and Rv1418 are separated by 24 nucleotides Rv1418 and Rv1419 are separated by 179 nucleotides Rv1419 and Rv1420 are separated by 63 nucleotides
Rv1415: Rv1415 - Probable riboflavin biosynthesis protein RibA2 : GTP cyclohydrolase II + 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase), at 1,590,397 to 1,591,674
Rv1415
Rv1416: Rv1416 - Probable riboflavin synthase beta chain RibH (6,7-dimethyl-8-ribityllumazine synthase) (DMRL synthase) (lumazine synthase), at 1,591,671 to 1,592,153
Rv1416
Rv1417: Rv1417 - Possible conserved membrane protein, at 1,592,150 to 1,592,614
Rv1417
Rv1418: Rv1418 - Probable lipoprotein LprH, at 1,592,639 to 1,593,325
Rv1418
Rv1419: Rv1419 - Unknown protein, at 1,593,505 to 1,593,978
Rv1419
Rv1420: Rv1420 - Probable excinuclease ABC (subunit C-nuclease) UvrC, at 1,594,042 to 1,595,982
Rv1420
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1592.505 kb on - strand, within Rv1417 at 1592.628 kb on - strand at 1592.876 kb on + strand, within Rv1418 at 1592.876 kb on + strand, within Rv1418 at 1592.975 kb on + strand, within Rv1418 at 1592.976 kb on - strand, within Rv1418 at 1592.978 kb on - strand, within Rv1418 at 1592.978 kb on - strand, within Rv1418 at 1593.171 kb on + strand, within Rv1418 at 1593.171 kb on + strand, within Rv1418 at 1593.172 kb on - strand, within Rv1418 at 1593.515 kb on + strand at 1593.515 kb on + strand at 1593.516 kb on - strand at 1594.031 kb on - strand at 1594.035 kb on + strand at 1594.036 kb on - strand at 1594.036 kb on - strand at 1594.036 kb on - strand at 1594.039 kb on + strand at 1594.039 kb on + strand at 1594.039 kb on + strand at 1594.135 kb on + strand at 1594.178 kb on - strand at 1594.288 kb on + strand, within Rv1420
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with 8-hydroxy-nitroquinoline 0.03 mM remove 1,592,505 - Rv1417 0.76 -0.9 1,592,628 - -1.1 1,592,876 + Rv1418 0.34 +1.9 1,592,876 + Rv1418 0.34 -0.7 1,592,975 + Rv1418 0.49 -0.8 1,592,976 - Rv1418 0.49 -0.5 1,592,978 - Rv1418 0.49 +0.3 1,592,978 - Rv1418 0.49 -0.6 1,593,171 + Rv1418 0.77 +0.3 1,593,171 + Rv1418 0.77 +0.5 1,593,172 - Rv1418 0.78 -1.1 1,593,515 + -0.8 1,593,515 + -0.1 1,593,516 - -1.9 1,594,031 - +0.0 1,594,035 + +0.0 1,594,036 - -1.0 1,594,036 - +1.9 1,594,036 - +1.9 1,594,039 + -0.1 1,594,039 + -1.7 1,594,039 + -0.7 1,594,135 + -0.4 1,594,178 - -0.6 1,594,288 + Rv1420 0.13 +0.9
Or see this region's nucleotide sequence