Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1346

Experiment: 7H9 with Doxycycline hyclate 0.0035 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1345 and Rv1346 overlap by 1 nucleotidesRv1346 and Rv1347c overlap by 34 nucleotidesRv1347c and GFF1405 are separated by 122 nucleotides Rv1345: Rv1345 - Probable fatty acyl-AMP ligase MbtM, at 1,509,281 to 1,510,846 Rv1345 Rv1346: Rv1346 - Acyl-CoA dehydrogenase MbtN, at 1,510,846 to 1,512,006 Rv1346 Rv1347c: Rv1347c - Lysine N-acetyltransferase MbtK, at 1,511,973 to 1,512,605 Rv1347c GFF1405 - tRNA-Leu, at 1,512,728 to 1,512,811 GFF1405 Position (kb) 1510 1511 1512 1513Strain fitness (log2 ratio) -1 0 1at 1509.880 kb on - strand, within Rv1345at 1510.061 kb on + strand, within Rv1345at 1510.062 kb on - strand, within Rv1345at 1510.205 kb on + strand, within Rv1345at 1510.297 kb on + strand, within Rv1345at 1510.338 kb on + strand, within Rv1345at 1510.339 kb on - strand, within Rv1345at 1510.991 kb on + strand, within Rv1346at 1511.146 kb on + strand, within Rv1346at 1511.252 kb on + strand, within Rv1346at 1511.253 kb on - strand, within Rv1346at 1511.368 kb on - strand, within Rv1346at 1511.682 kb on + strand, within Rv1346at 1511.848 kb on + strand, within Rv1346at 1512.712 kb on + strandat 1512.892 kb on + strandat 1512.907 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Doxycycline hyclate 0.0035 mM
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1,509,880 - Rv1345 0.38 +0.2
1,510,061 + Rv1345 0.50 -1.0
1,510,062 - Rv1345 0.50 -0.1
1,510,205 + Rv1345 0.59 -0.3
1,510,297 + Rv1345 0.65 -0.0
1,510,338 + Rv1345 0.67 -0.1
1,510,339 - Rv1345 0.68 -0.5
1,510,991 + Rv1346 0.12 -1.1
1,511,146 + Rv1346 0.26 -0.9
1,511,252 + Rv1346 0.35 -0.2
1,511,253 - Rv1346 0.35 -0.6
1,511,368 - Rv1346 0.45 -1.2
1,511,682 + Rv1346 0.72 -1.7
1,511,848 + Rv1346 0.86 -0.4
1,512,712 + -1.5
1,512,892 + +0.1
1,512,907 + +1.3

Or see this region's nucleotide sequence