Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3719 and Rv3720 are separated by 17 nucleotides Rv3720 and Rv3721c overlap by 4 nucleotides
Rv3719: Rv3719 - Conserved protein, at 4,162,306 to 4,163,718
Rv3719
Rv3720: Rv3720 - Possible fatty acid synthase, at 4,163,736 to 4,164,998
Rv3720
Rv3721c: Rv3721c - DNA polymerase III (subunit gamma/tau) DnaZ/X, at 4,164,995 to 4,166,731
Rv3721c
Position (kb) 4163
4164
4165 Strain fitness (log2 ratio) -2
-1
0
1 at 4162.789 kb on + strand, within Rv3719 at 4162.970 kb on - strand, within Rv3719 at 4162.970 kb on - strand, within Rv3719 at 4163.005 kb on + strand, within Rv3719 at 4163.006 kb on - strand, within Rv3719 at 4163.053 kb on + strand, within Rv3719 at 4163.071 kb on + strand, within Rv3719 at 4163.072 kb on - strand, within Rv3719 at 4163.072 kb on - strand, within Rv3719 at 4163.131 kb on + strand, within Rv3719 at 4163.472 kb on + strand, within Rv3719 at 4163.477 kb on - strand, within Rv3719 at 4163.477 kb on - strand, within Rv3719 at 4163.599 kb on + strand at 4163.642 kb on - strand at 4163.642 kb on - strand at 4163.663 kb on - strand at 4163.872 kb on + strand, within Rv3720 at 4163.873 kb on - strand, within Rv3720 at 4163.911 kb on - strand, within Rv3720 at 4163.941 kb on + strand, within Rv3720 at 4163.941 kb on + strand, within Rv3720 at 4163.941 kb on + strand, within Rv3720 at 4163.942 kb on - strand, within Rv3720 at 4164.055 kb on + strand, within Rv3720 at 4164.163 kb on - strand, within Rv3720 at 4164.213 kb on + strand, within Rv3720 at 4164.274 kb on - strand, within Rv3720 at 4164.320 kb on - strand, within Rv3720 at 4164.564 kb on + strand, within Rv3720 at 4164.564 kb on + strand, within Rv3720 at 4164.713 kb on + strand, within Rv3720 at 4164.716 kb on + strand, within Rv3720 at 4164.717 kb on - strand, within Rv3720 at 4164.876 kb on + strand at 4164.876 kb on + strand at 4164.877 kb on - strand at 4164.877 kb on - strand at 4164.922 kb on + strand at 4164.931 kb on + strand at 4164.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 4,162,789 + Rv3719 0.34 -0.6 4,162,970 - Rv3719 0.47 -0.4 4,162,970 - Rv3719 0.47 -0.5 4,163,005 + Rv3719 0.49 +0.6 4,163,006 - Rv3719 0.50 -0.4 4,163,053 + Rv3719 0.53 +1.6 4,163,071 + Rv3719 0.54 +0.9 4,163,072 - Rv3719 0.54 +0.2 4,163,072 - Rv3719 0.54 +0.3 4,163,131 + Rv3719 0.58 -0.1 4,163,472 + Rv3719 0.83 -2.4 4,163,477 - Rv3719 0.83 -0.1 4,163,477 - Rv3719 0.83 +0.0 4,163,599 + -1.9 4,163,642 - -0.9 4,163,642 - -0.4 4,163,663 - -0.3 4,163,872 + Rv3720 0.11 +1.7 4,163,873 - Rv3720 0.11 -1.7 4,163,911 - Rv3720 0.14 -1.1 4,163,941 + Rv3720 0.16 -1.0 4,163,941 + Rv3720 0.16 +0.6 4,163,941 + Rv3720 0.16 -0.9 4,163,942 - Rv3720 0.16 -0.8 4,164,055 + Rv3720 0.25 -1.4 4,164,163 - Rv3720 0.34 -1.1 4,164,213 + Rv3720 0.38 -0.4 4,164,274 - Rv3720 0.43 -1.0 4,164,320 - Rv3720 0.46 -2.6 4,164,564 + Rv3720 0.66 -1.2 4,164,564 + Rv3720 0.66 -1.6 4,164,713 + Rv3720 0.77 +0.2 4,164,716 + Rv3720 0.78 -0.4 4,164,717 - Rv3720 0.78 -1.0 4,164,876 + +0.4 4,164,876 + -0.7 4,164,877 - -0.1 4,164,877 - +0.0 4,164,922 + -1.3 4,164,931 + -0.1 4,164,931 + -0.3
Or see this region's nucleotide sequence