Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3651 and Rv3652 are separated by 753 nucleotides Rv3652 and Rv3653 overlap by 7 nucleotides Rv3653 and Rv3654c are separated by 132 nucleotides Rv3654c and Rv3655c are separated by 8 nucleotides
Rv3651: Rv3651 - Conserved hypothetical protein, at 4,091,841 to 4,092,878
Rv3651
Rv3652: Rv3652 - PE-PGRS family-related protein PE_PGRS60, at 4,093,632 to 4,093,946
Rv3652
Rv3653: Rv3653 - PE-PGRS family-related protein PE_PGRS61, at 4,093,940 to 4,094,527
Rv3653
Rv3654c: Rv3654c - Conserved hypothetical protein, at 4,094,660 to 4,094,914
Rv3654c
Rv3655c: Rv3655c - Conserved hypothetical protein, at 4,094,923 to 4,095,300
Rv3655c
Position (kb)
4093
4094 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4093.553 kb on + strand at 4093.638 kb on + strand at 4093.638 kb on + strand at 4093.638 kb on + strand at 4093.792 kb on - strand, within Rv3652 at 4093.814 kb on + strand, within Rv3652 at 4093.814 kb on + strand, within Rv3652 at 4093.815 kb on - strand, within Rv3652 at 4094.562 kb on - strand at 4094.585 kb on - strand at 4094.613 kb on - strand at 4094.613 kb on - strand at 4094.650 kb on + strand at 4094.924 kb on + strand at 4094.924 kb on + strand at 4094.929 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 4,093,553 + +1.7 4,093,638 + +0.6 4,093,638 + -0.3 4,093,638 + -0.3 4,093,792 - Rv3652 0.51 -0.4 4,093,814 + Rv3652 0.58 +1.4 4,093,814 + Rv3652 0.58 -0.3 4,093,815 - Rv3652 0.58 +2.9 4,094,562 - -0.5 4,094,585 - +0.5 4,094,613 - -0.6 4,094,613 - +0.2 4,094,650 + +0.9 4,094,924 + +1.1 4,094,924 + -0.2 4,094,929 - -0.4
Or see this region's nucleotide sequence