Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3568c and Rv3569c overlap by 4 nucleotides Rv3569c and Rv3570c are separated by 14 nucleotides Rv3570c and Rv3571 are separated by 146 nucleotides
Rv3568c: Rv3568c - 3,4-DHSA dioxygenase, at 4,009,297 to 4,010,199
Rv3568c
Rv3569c: Rv3569c - 4,9-DHSA hydrolase, at 4,010,196 to 4,011,071
Rv3569c
Rv3570c: Rv3570c - Possible oxidoreductase. Possible 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione hydroxylase., at 4,011,086 to 4,012,270
Rv3570c
Rv3571: Rv3571 - Reductase component of 3-ketosteroid-9-alpha-hydroxylase KshB, at 4,012,417 to 4,013,493
Rv3571
Position (kb)
4011
4012
4013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4010.283 kb on + strand at 4010.283 kb on + strand at 4010.642 kb on + strand, within Rv3569c at 4010.643 kb on - strand, within Rv3569c at 4010.693 kb on - strand, within Rv3569c at 4010.989 kb on + strand at 4011.062 kb on + strand at 4011.141 kb on + strand at 4011.141 kb on + strand at 4011.584 kb on - strand, within Rv3570c at 4011.710 kb on + strand, within Rv3570c at 4011.710 kb on + strand, within Rv3570c at 4011.711 kb on - strand, within Rv3570c at 4011.723 kb on + strand, within Rv3570c at 4011.723 kb on + strand, within Rv3570c at 4011.724 kb on - strand, within Rv3570c at 4012.272 kb on + strand at 4012.272 kb on + strand at 4012.342 kb on - strand at 4012.615 kb on - strand, within Rv3571 at 4012.960 kb on + strand, within Rv3571 at 4012.961 kb on - strand, within Rv3571 at 4013.048 kb on - strand, within Rv3571
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 4,010,283 + -3.7 4,010,283 + -2.2 4,010,642 + Rv3569c 0.51 -2.4 4,010,643 - Rv3569c 0.51 -1.2 4,010,693 - Rv3569c 0.57 +1.9 4,010,989 + +0.4 4,011,062 + -1.1 4,011,141 + -0.2 4,011,141 + -3.0 4,011,584 - Rv3570c 0.42 -3.2 4,011,710 + Rv3570c 0.53 -1.5 4,011,710 + Rv3570c 0.53 -1.5 4,011,711 - Rv3570c 0.53 -2.2 4,011,723 + Rv3570c 0.54 -2.5 4,011,723 + Rv3570c 0.54 -1.8 4,011,724 - Rv3570c 0.54 -3.1 4,012,272 + -3.3 4,012,272 + -2.9 4,012,342 - +1.3 4,012,615 - Rv3571 0.18 -0.1 4,012,960 + Rv3571 0.50 -2.9 4,012,961 - Rv3571 0.51 -1.3 4,013,048 - Rv3571 0.59 -2.5
Or see this region's nucleotide sequence