Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3371

Experiment: cholesterol 100uM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3370c and Rv3371 are separated by 191 nucleotidesRv3371 and Rv3372 are separated by 41 nucleotides Rv3370c: Rv3370c - Probable DNA polymerase III (alpha chain) DnaE2 (DNA nucleotidyltransferase), at 3,781,501 to 3,784,740 Rv3370c Rv3371: Rv3371 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 3,784,932 to 3,786,272 Rv3371 Rv3372: Rv3372 - Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP), at 3,786,314 to 3,787,489 Rv3372 Position (kb) 3784 3785 3786 3787Strain fitness (log2 ratio) -2 -1 0 1at 3784.604 kb on + strandat 3784.895 kb on + strandat 3784.895 kb on + strandat 3784.896 kb on - strandat 3784.896 kb on - strandat 3784.896 kb on - strandat 3785.096 kb on + strand, within Rv3371at 3785.858 kb on + strand, within Rv3371at 3786.085 kb on - strand, within Rv3371at 3786.085 kb on - strand, within Rv3371at 3786.179 kb on + strandat 3786.179 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction cholesterol 100uM (C)
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3,784,604 + -2.0
3,784,895 + -1.2
3,784,895 + -0.4
3,784,896 - +0.2
3,784,896 - -0.5
3,784,896 - +0.3
3,785,096 + Rv3371 0.12 -1.1
3,785,858 + Rv3371 0.69 -1.6
3,786,085 - Rv3371 0.86 -0.6
3,786,085 - Rv3371 0.86 -0.4
3,786,179 + -0.3
3,786,179 + -0.9

Or see this region's nucleotide sequence