Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3322 and Rv3192 are separated by 119 nucleotides Rv3192 and Rv3193c are separated by 169 nucleotides Rv3193c and Rv3194c are separated by 91 nucleotides
GFF3322 - tRNA-Met, at 3,559,370 to 3,559,443
GFF3322
Rv3192: Rv3192 - Conserved hypothetical alanine and proline-rich protein, at 3,559,563 to 3,560,024
Rv3192
Rv3193c: Rv3193c - Probable conserved transmembrane protein, at 3,560,194 to 3,563,172
Rv3193c
Rv3194c: Rv3194c - Possible conserved secreted protein, at 3,563,264 to 3,564,286
Rv3194c
Position (kb)
3560
3561
3562
3563
3564 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3559.195 kb on - strand at 3559.296 kb on - strand at 3559.533 kb on + strand at 3559.534 kb on - strand at 3559.775 kb on + strand, within Rv3192 at 3559.776 kb on - strand, within Rv3192 at 3559.837 kb on - strand, within Rv3192 at 3559.841 kb on + strand, within Rv3192 at 3559.875 kb on + strand, within Rv3192 at 3559.876 kb on - strand, within Rv3192 at 3560.341 kb on + strand at 3560.615 kb on + strand, within Rv3193c at 3560.940 kb on - strand, within Rv3193c at 3561.612 kb on + strand, within Rv3193c at 3562.204 kb on - strand, within Rv3193c at 3562.479 kb on - strand, within Rv3193c at 3563.266 kb on - strand at 3563.323 kb on + strand at 3563.643 kb on - strand, within Rv3194c at 3563.671 kb on + strand, within Rv3194c at 3563.671 kb on + strand, within Rv3194c at 3563.672 kb on - strand, within Rv3194c at 3563.916 kb on + strand, within Rv3194c at 3563.994 kb on + strand, within Rv3194c at 3564.062 kb on + strand, within Rv3194c at 3564.063 kb on - strand, within Rv3194c at 3564.114 kb on + strand, within Rv3194c at 3564.155 kb on + strand, within Rv3194c
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 3,559,195 - +0.7 3,559,296 - -0.1 3,559,533 + -1.3 3,559,534 - +0.3 3,559,775 + Rv3192 0.46 -0.6 3,559,776 - Rv3192 0.46 -0.3 3,559,837 - Rv3192 0.59 -0.2 3,559,841 + Rv3192 0.60 -2.9 3,559,875 + Rv3192 0.68 -0.3 3,559,876 - Rv3192 0.68 -1.4 3,560,341 + +1.3 3,560,615 + Rv3193c 0.14 -0.1 3,560,940 - Rv3193c 0.25 +0.5 3,561,612 + Rv3193c 0.48 -1.2 3,562,204 - Rv3193c 0.67 -1.6 3,562,479 - Rv3193c 0.77 +1.9 3,563,266 - -0.0 3,563,323 + +0.3 3,563,643 - Rv3194c 0.37 -1.0 3,563,671 + Rv3194c 0.40 +0.2 3,563,671 + Rv3194c 0.40 +0.4 3,563,672 - Rv3194c 0.40 +1.1 3,563,916 + Rv3194c 0.64 -0.2 3,563,994 + Rv3194c 0.71 -0.0 3,564,062 + Rv3194c 0.78 -0.3 3,564,063 - Rv3194c 0.78 -0.1 3,564,114 + Rv3194c 0.83 -0.8 3,564,155 + Rv3194c 0.87 +0.2
Or see this region's nucleotide sequence