Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3156 and Rv3157 overlap by 4 nucleotides Rv3157 and Rv3158 overlap by 4 nucleotides
Rv3156: Rv3156 - Probable NADH dehydrogenase I (chain L) NuoL (NADH-ubiquinone oxidoreductase chain L), at 3,522,234 to 3,524,135
Rv3156
Rv3157: Rv3157 - Probable NADH dehydrogenase I (chain M) NUOK (NADH-ubiquinone oxidoreductase chain M), at 3,524,132 to 3,525,793
Rv3157
Rv3158: Rv3158 - Probable NADH dehydrogenase I (chain N) NuoN (NADH-ubiquinone oxidoreductase chain N), at 3,525,790 to 3,527,385
Rv3158
Position (kb)
3524
3525
3526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3523.285 kb on + strand, within Rv3156 at 3523.821 kb on + strand, within Rv3156 at 3523.822 kb on - strand, within Rv3156 at 3524.558 kb on + strand, within Rv3157 at 3524.558 kb on + strand, within Rv3157 at 3524.777 kb on + strand, within Rv3157 at 3525.114 kb on - strand, within Rv3157 at 3525.246 kb on - strand, within Rv3157 at 3525.519 kb on - strand, within Rv3157 at 3525.533 kb on + strand, within Rv3157 at 3525.633 kb on + strand at 3525.817 kb on + strand at 3526.055 kb on + strand, within Rv3158 at 3526.055 kb on + strand, within Rv3158 at 3526.055 kb on + strand, within Rv3158 at 3526.056 kb on - strand, within Rv3158 at 3526.408 kb on + strand, within Rv3158 at 3526.572 kb on - strand, within Rv3158 at 3526.572 kb on - strand, within Rv3158 at 3526.572 kb on - strand, within Rv3158 at 3526.574 kb on + strand, within Rv3158 at 3526.574 kb on + strand, within Rv3158 at 3526.575 kb on - strand, within Rv3158 at 3526.575 kb on - strand, within Rv3158 at 3526.575 kb on - strand, within Rv3158 at 3526.705 kb on + strand, within Rv3158 at 3526.706 kb on - strand, within Rv3158
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 3,523,285 + Rv3156 0.55 -2.8 3,523,821 + Rv3156 0.83 -0.6 3,523,822 - Rv3156 0.83 +0.6 3,524,558 + Rv3157 0.26 -1.0 3,524,558 + Rv3157 0.26 -1.6 3,524,777 + Rv3157 0.39 -0.8 3,525,114 - Rv3157 0.59 -0.9 3,525,246 - Rv3157 0.67 -1.0 3,525,519 - Rv3157 0.83 -0.8 3,525,533 + Rv3157 0.84 -0.7 3,525,633 + +0.5 3,525,817 + -0.7 3,526,055 + Rv3158 0.17 +1.8 3,526,055 + Rv3158 0.17 +0.2 3,526,055 + Rv3158 0.17 -0.1 3,526,056 - Rv3158 0.17 +0.5 3,526,408 + Rv3158 0.39 -1.2 3,526,572 - Rv3158 0.49 -0.4 3,526,572 - Rv3158 0.49 -0.8 3,526,572 - Rv3158 0.49 +1.4 3,526,574 + Rv3158 0.49 -0.8 3,526,574 + Rv3158 0.49 -1.4 3,526,575 - Rv3158 0.49 -0.9 3,526,575 - Rv3158 0.49 -1.2 3,526,575 - Rv3158 0.49 -1.1 3,526,705 + Rv3158 0.57 -2.0 3,526,706 - Rv3158 0.57 -3.2
Or see this region's nucleotide sequence