Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2250c

Experiment: cholesterol 100uM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2249c and Rv2250c overlap by 7 nucleotidesRv2250c and Rv2250A are separated by 47 nucleotidesRv2250A and Rv2251 overlap by 257 nucleotides Rv2249c: Rv2249c - Probable glycerol-3-phosphate dehydrogenase GlpD1, at 2,523,241 to 2,524,791 Rv2249c Rv2250c: Rv2250c - Possible transcriptional regulatory protein, at 2,524,785 to 2,525,354 Rv2250c Rv2250A: Rv2250A - Possible flavoprotein, at 2,525,402 to 2,525,821 Rv2250A Rv2251: Rv2251 - Possible flavoprotein, at 2,525,565 to 2,526,992 Rv2251 Position (kb) 2524 2525 2526Strain fitness (log2 ratio) -3 -2 -1 0 1at 2523.816 kb on + strand, within Rv2249cat 2524.560 kb on - strand, within Rv2249cat 2524.663 kb on + strandat 2524.795 kb on + strandat 2524.796 kb on - strandat 2524.796 kb on - strandat 2524.815 kb on - strandat 2525.020 kb on + strand, within Rv2250cat 2525.103 kb on + strand, within Rv2250cat 2525.594 kb on + strandat 2525.862 kb on - strand, within Rv2251at 2526.250 kb on - strand, within Rv2251at 2526.250 kb on - strand, within Rv2251

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Per-strain Table

Position Strand Gene LocusTag Fraction cholesterol 100uM (C)
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2,523,816 + Rv2249c 0.37 -0.5
2,524,560 - Rv2249c 0.85 +0.4
2,524,663 + +0.8
2,524,795 + -0.2
2,524,796 - -0.3
2,524,796 - -2.9
2,524,815 - -0.1
2,525,020 + Rv2250c 0.41 -1.2
2,525,103 + Rv2250c 0.56 -0.9
2,525,594 + -0.7
2,525,862 - Rv2251 0.21 +0.8
2,526,250 - Rv2251 0.48 +0.3
2,526,250 - Rv2251 0.48 -0.3

Or see this region's nucleotide sequence