Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2101 and Rv2102 are separated by 109 nucleotides Rv2102 and Rv2103c overlap by 22 nucleotides Rv2103c and Rv2104c are separated by 6 nucleotides
Rv2101: Rv2101 - Probable helicase HelZ, at 2,360,240 to 2,363,281
Rv2101
Rv2102: Rv2102 - Conserved hypothetical protein, at 2,363,391 to 2,364,107
Rv2102
Rv2103c: Rv2103c - Possible toxin VapC37. Contains PIN domain., at 2,364,086 to 2,364,520
Rv2103c
Rv2104c: Rv2104c - Possible antitoxin VapB37, at 2,364,527 to 2,364,781
Rv2104c
Position (kb)
2363
2364
2365 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2362.475 kb on + strand, within Rv2101 at 2362.476 kb on - strand, within Rv2101 at 2362.476 kb on - strand, within Rv2101 at 2362.547 kb on + strand, within Rv2101 at 2362.580 kb on + strand, within Rv2101 at 2362.581 kb on - strand, within Rv2101 at 2362.883 kb on + strand, within Rv2101 at 2363.279 kb on + strand at 2363.279 kb on + strand at 2363.289 kb on + strand at 2363.290 kb on - strand at 2363.290 kb on - strand at 2363.439 kb on + strand at 2363.440 kb on - strand at 2363.440 kb on - strand at 2363.701 kb on + strand, within Rv2102 at 2363.763 kb on + strand, within Rv2102 at 2363.763 kb on + strand, within Rv2102 at 2363.764 kb on - strand, within Rv2102 at 2363.937 kb on + strand, within Rv2102 at 2363.937 kb on + strand, within Rv2102 at 2364.066 kb on + strand at 2364.067 kb on - strand at 2364.147 kb on + strand, within Rv2103c at 2364.147 kb on + strand, within Rv2103c at 2364.148 kb on - strand, within Rv2103c at 2364.228 kb on + strand, within Rv2103c at 2364.228 kb on + strand, within Rv2103c at 2364.228 kb on + strand, within Rv2103c at 2364.228 kb on + strand, within Rv2103c at 2364.229 kb on - strand, within Rv2103c at 2364.490 kb on - strand at 2364.528 kb on + strand at 2364.529 kb on - strand at 2364.845 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 2,362,475 + Rv2101 0.73 +1.8 2,362,476 - Rv2101 0.74 -0.4 2,362,476 - Rv2101 0.74 +0.1 2,362,547 + Rv2101 0.76 +0.1 2,362,580 + Rv2101 0.77 -0.7 2,362,581 - Rv2101 0.77 -1.2 2,362,883 + Rv2101 0.87 +1.8 2,363,279 + +0.2 2,363,279 + +0.2 2,363,289 + +0.2 2,363,290 - +0.5 2,363,290 - -0.2 2,363,439 + -0.4 2,363,440 - +0.3 2,363,440 - -0.3 2,363,701 + Rv2102 0.43 -1.5 2,363,763 + Rv2102 0.52 -1.1 2,363,763 + Rv2102 0.52 -0.3 2,363,764 - Rv2102 0.52 -1.1 2,363,937 + Rv2102 0.76 -2.9 2,363,937 + Rv2102 0.76 -0.7 2,364,066 + +0.1 2,364,067 - -0.2 2,364,147 + Rv2103c 0.14 +0.1 2,364,147 + Rv2103c 0.14 -0.8 2,364,148 - Rv2103c 0.14 -0.2 2,364,228 + Rv2103c 0.33 -0.3 2,364,228 + Rv2103c 0.33 -1.0 2,364,228 + Rv2103c 0.33 -0.4 2,364,228 + Rv2103c 0.33 +0.2 2,364,229 - Rv2103c 0.33 +0.1 2,364,490 - +0.3 2,364,528 + +0.1 2,364,529 - -0.8 2,364,845 - -0.8
Or see this region's nucleotide sequence