Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2048c and Rv2049c are separated by 306 nucleotides Rv2049c and Rv2050 are separated by 303 nucleotides Rv2050 and Rv2051c overlap by 26 nucleotides
Rv2048c: Rv2048c - Polyketide synthase Pks12, at 2,294,531 to 2,306,986
Rv2048c
Rv2049c: Rv2049c - Conserved hypothetical protein, at 2,307,293 to 2,307,517
Rv2049c
Rv2050: Rv2050 - Conserved protein, at 2,307,821 to 2,308,156
Rv2050
Rv2051c: Rv2051c - Polyprenol-monophosphomannose synthase Ppm1, at 2,308,131 to 2,310,755
Rv2051c
Position (kb)
2307
2308 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2306.473 kb on - strand at 2306.709 kb on + strand at 2306.710 kb on - strand at 2306.716 kb on + strand at 2306.988 kb on - strand at 2306.990 kb on + strand at 2306.991 kb on - strand at 2306.991 kb on - strand at 2307.000 kb on + strand at 2307.001 kb on - strand at 2307.010 kb on + strand at 2307.014 kb on - strand at 2307.014 kb on - strand at 2307.117 kb on + strand at 2307.117 kb on + strand at 2307.118 kb on - strand at 2307.153 kb on - strand at 2307.254 kb on - strand at 2307.294 kb on + strand at 2307.429 kb on + strand, within Rv2049c at 2307.531 kb on + strand at 2307.561 kb on + strand at 2307.656 kb on + strand at 2307.656 kb on + strand at 2307.656 kb on + strand at 2307.657 kb on - strand at 2307.657 kb on - strand at 2307.723 kb on + strand at 2307.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 2,306,473 - -0.7 2,306,709 + -0.2 2,306,710 - -1.0 2,306,716 + +0.4 2,306,988 - -0.5 2,306,990 + -0.5 2,306,991 - -0.2 2,306,991 - -0.3 2,307,000 + +0.6 2,307,001 - -2.6 2,307,010 + -1.9 2,307,014 - -1.3 2,307,014 - -0.3 2,307,117 + +0.2 2,307,117 + -0.1 2,307,118 - +0.8 2,307,153 - +0.4 2,307,254 - -2.7 2,307,294 + -0.7 2,307,429 + Rv2049c 0.60 -1.0 2,307,531 + -0.3 2,307,561 + +1.1 2,307,656 + -0.2 2,307,656 + +0.2 2,307,656 + -1.5 2,307,657 - -1.1 2,307,657 - +0.3 2,307,723 + +0.7 2,307,776 + +2.8
Or see this region's nucleotide sequence