Experiment: cholesterol 100uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1406 and Rv1407 overlap by 4 nucleotides Rv1407 and Rv1408 are separated by 24 nucleotides Rv1408 and Rv1409 overlap by 4 nucleotides
Rv1406: Rv1406 - Probable methionyl-tRNA formyltransferase Fmt, at 1,582,166 to 1,583,104
Rv1406
Rv1407: Rv1407 - Probable Fmu protein (sun protein), at 1,583,101 to 1,584,474
Rv1407
Rv1408: Rv1408 - Probable ribulose-phosphate 3-epimerase Rpe (PPE) (R5P3E) (pentose-5-phosphate 3-epimerase), at 1,584,499 to 1,585,197
Rv1408
Rv1409: Rv1409 - Probable bifunctional riboflavin biosynthesis protein RibG : diaminohydroxyphosphoribosylaminopyrimidine deaminase (riboflavin-specific deaminase) + 5-amino-6-(5-phosphoribosylamino) uracil reductase (HTP reductase), at 1,585,194 to 1,586,213
Rv1409
Position (kb)
1583
1584
1585 Strain fitness (log2 ratio)
-2
-1
0
1 at 1583.278 kb on + strand, within Rv1407 at 1583.367 kb on - strand, within Rv1407 at 1583.367 kb on - strand, within Rv1407 at 1583.389 kb on + strand, within Rv1407 at 1583.786 kb on - strand, within Rv1407 at 1583.904 kb on + strand, within Rv1407 at 1584.030 kb on - strand, within Rv1407 at 1584.149 kb on + strand, within Rv1407 at 1584.194 kb on + strand, within Rv1407 at 1584.195 kb on - strand, within Rv1407 at 1584.195 kb on - strand, within Rv1407 at 1584.213 kb on - strand, within Rv1407 at 1584.213 kb on - strand, within Rv1407 at 1584.271 kb on + strand, within Rv1407 at 1584.271 kb on + strand, within Rv1407 at 1584.272 kb on - strand, within Rv1407 at 1584.272 kb on - strand, within Rv1407 at 1584.272 kb on - strand, within Rv1407 at 1584.272 kb on - strand, within Rv1407 at 1584.495 kb on + strand at 1584.495 kb on + strand at 1584.496 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction cholesterol 100uM (C) remove 1,583,278 + Rv1407 0.13 -0.5 1,583,367 - Rv1407 0.19 -0.4 1,583,367 - Rv1407 0.19 -0.3 1,583,389 + Rv1407 0.21 -0.7 1,583,786 - Rv1407 0.50 -0.2 1,583,904 + Rv1407 0.58 -1.8 1,584,030 - Rv1407 0.68 -0.5 1,584,149 + Rv1407 0.76 -0.6 1,584,194 + Rv1407 0.80 -0.4 1,584,195 - Rv1407 0.80 -1.6 1,584,195 - Rv1407 0.80 -0.8 1,584,213 - Rv1407 0.81 -0.4 1,584,213 - Rv1407 0.81 -0.5 1,584,271 + Rv1407 0.85 -0.0 1,584,271 + Rv1407 0.85 -0.7 1,584,272 - Rv1407 0.85 +0.5 1,584,272 - Rv1407 0.85 -1.4 1,584,272 - Rv1407 0.85 -0.0 1,584,272 - Rv1407 0.85 -0.2 1,584,495 + -0.3 1,584,495 + +1.2 1,584,496 - -0.9
Or see this region's nucleotide sequence