Experiment: oleate 200uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3848 and Rv3849 are separated by 264 nucleotides Rv3849 and Rv3850 are separated by 117 nucleotides Rv3850 and Rv3851 are separated by 11 nucleotides Rv3851 and Rv3852 are separated by 106 nucleotides Rv3852 and Rv3853 are separated by 16 nucleotides
Rv3848: Rv3848 - Probable conserved transmembrane protein, at 4,322,326 to 4,323,234
Rv3848
Rv3849: Rv3849 - ESX-1 transcriptional regulatory protein EspR, at 4,323,499 to 4,323,897
Rv3849
Rv3850: Rv3850 - Conserved protein, at 4,324,015 to 4,324,671
Rv3850
Rv3851: Rv3851 - Possible membrane protein, at 4,324,683 to 4,324,967
Rv3851
Rv3852: Rv3852 - Possible histone-like protein Hns, at 4,325,074 to 4,325,478
Rv3852
Rv3853: Rv3853 - Regulator of RNase E activity a RraA, at 4,325,495 to 4,325,968
Rv3853
Position (kb)
4324
4325 Strain fitness (log2 ratio)
-2
-1
0
1 at 4323.297 kb on + strand at 4323.547 kb on + strand, within Rv3849 at 4323.896 kb on - strand at 4323.959 kb on - strand at 4324.235 kb on + strand, within Rv3850 at 4324.326 kb on - strand, within Rv3850 at 4324.327 kb on + strand, within Rv3850 at 4324.670 kb on - strand at 4325.038 kb on + strand at 4325.133 kb on + strand, within Rv3852 at 4325.229 kb on + strand, within Rv3852 at 4325.229 kb on + strand, within Rv3852 at 4325.229 kb on + strand, within Rv3852 at 4325.230 kb on - strand, within Rv3852 at 4325.230 kb on - strand, within Rv3852 at 4325.230 kb on - strand, within Rv3852 at 4325.230 kb on - strand, within Rv3852 at 4325.230 kb on - strand, within Rv3852 at 4325.493 kb on + strand at 4325.493 kb on + strand at 4325.494 kb on - strand at 4325.565 kb on + strand, within Rv3853 at 4325.565 kb on + strand, within Rv3853 at 4325.566 kb on - strand, within Rv3853
Per-strain Table
Position Strand Gene LocusTag Fraction oleate 200uM (C) remove 4,323,297 + +1.1 4,323,547 + Rv3849 0.12 -0.2 4,323,896 - +0.5 4,323,959 - -0.4 4,324,235 + Rv3850 0.33 +1.4 4,324,326 - Rv3850 0.47 +0.6 4,324,327 + Rv3850 0.47 -1.6 4,324,670 - +0.2 4,325,038 + +0.7 4,325,133 + Rv3852 0.15 -2.0 4,325,229 + Rv3852 0.38 +0.8 4,325,229 + Rv3852 0.38 -0.4 4,325,229 + Rv3852 0.38 -0.4 4,325,230 - Rv3852 0.39 -0.1 4,325,230 - Rv3852 0.39 -0.5 4,325,230 - Rv3852 0.39 -0.4 4,325,230 - Rv3852 0.39 +0.3 4,325,230 - Rv3852 0.39 -0.4 4,325,493 + -0.2 4,325,493 + -1.0 4,325,494 - -1.1 4,325,565 + Rv3853 0.15 -1.1 4,325,565 + Rv3853 0.15 +0.5 4,325,566 - Rv3853 0.15 -0.5
Or see this region's nucleotide sequence