Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1134

Experiment: oleate 200uM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1133c and Rv1134 are separated by 575 nucleotidesRv1134 and Rv1135c are separated by 113 nucleotides Rv1133c: Rv1133c - Probable 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase MetE (methionine synthase, vitamin-B12 independent isozyme), at 1,259,067 to 1,261,346 Rv1133c Rv1134: Rv1134 - Hypothetical protein, at 1,261,922 to 1,262,158 Rv1134 Rv1135c: Rv1135c - PPE family protein PPE16, at 1,262,272 to 1,264,128 Rv1135c Position (kb) 1261 1262 1263Strain fitness (log2 ratio) -1 0 1 2 3at 1261.723 kb on + strandat 1261.724 kb on - strandat 1261.862 kb on + strandat 1261.862 kb on + strandat 1261.862 kb on + strandat 1261.931 kb on + strandat 1262.111 kb on + strand, within Rv1134at 1262.111 kb on + strand, within Rv1134at 1262.111 kb on + strand, within Rv1134at 1262.111 kb on + strand, within Rv1134at 1262.111 kb on + strand, within Rv1134at 1262.112 kb on - strand, within Rv1134at 1262.556 kb on + strand, within Rv1135cat 1262.557 kb on - strand, within Rv1135cat 1262.557 kb on - strand, within Rv1135cat 1262.762 kb on + strand, within Rv1135cat 1262.762 kb on + strand, within Rv1135cat 1262.763 kb on - strand, within Rv1135c

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Per-strain Table

Position Strand Gene LocusTag Fraction oleate 200uM (C)
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1,261,723 + -0.9
1,261,724 - +3.0
1,261,862 + -0.1
1,261,862 + -1.1
1,261,862 + -0.5
1,261,931 + -0.2
1,262,111 + Rv1134 0.80 -1.0
1,262,111 + Rv1134 0.80 +0.6
1,262,111 + Rv1134 0.80 -1.2
1,262,111 + Rv1134 0.80 -0.7
1,262,111 + Rv1134 0.80 +0.0
1,262,112 - Rv1134 0.80 -0.2
1,262,556 + Rv1135c 0.15 +0.0
1,262,557 - Rv1135c 0.15 +0.3
1,262,557 - Rv1135c 0.15 -0.2
1,262,762 + Rv1135c 0.26 -0.1
1,262,762 + Rv1135c 0.26 +0.9
1,262,763 - Rv1135c 0.26 +0.1

Or see this region's nucleotide sequence