Experiment: Urea 10mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0183 and Rv0184 are separated by 41 nucleotides Rv0184 and Rv0185 overlap by 4 nucleotides Rv0185 and Rv0186 are separated by 44 nucleotides
Rv0183: Rv0183 - Possible lysophospholipase, at 214,088 to 214,927
Rv0183
Rv0184: Rv0184 - Conserved hypothetical protein, at 214,969 to 215,718
Rv0184
Rv0185: Rv0185 - Conserved hypothetical protein, at 215,715 to 216,224
Rv0185
Rv0186: Rv0186 - Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase), at 216,269 to 218,344
Rv0186
Position (kb)
215
216
217 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 214.744 kb on - strand, within Rv0183 at 214.744 kb on - strand, within Rv0183 at 215.200 kb on + strand, within Rv0184 at 215.200 kb on + strand, within Rv0184 at 215.318 kb on + strand, within Rv0184 at 215.319 kb on - strand, within Rv0184 at 215.319 kb on - strand, within Rv0184 at 215.320 kb on + strand, within Rv0184 at 215.761 kb on - strand at 215.895 kb on + strand, within Rv0185 at 215.895 kb on + strand, within Rv0185 at 215.896 kb on - strand, within Rv0185 at 216.020 kb on + strand, within Rv0185 at 216.021 kb on - strand, within Rv0185 at 216.076 kb on + strand, within Rv0185 at 216.076 kb on + strand, within Rv0185 at 216.202 kb on - strand at 216.378 kb on - strand at 216.378 kb on - strand at 216.869 kb on + strand, within Rv0186 at 217.101 kb on + strand, within Rv0186
Per-strain Table
Position Strand Gene LocusTag Fraction Urea 10mM (N) remove 214,744 - Rv0183 0.78 -0.6 214,744 - Rv0183 0.78 -0.3 215,200 + Rv0184 0.31 -0.1 215,200 + Rv0184 0.31 -1.9 215,318 + Rv0184 0.47 -0.1 215,319 - Rv0184 0.47 -0.2 215,319 - Rv0184 0.47 -0.5 215,320 + Rv0184 0.47 -1.9 215,761 - +0.2 215,895 + Rv0185 0.35 +0.7 215,895 + Rv0185 0.35 -0.3 215,896 - Rv0185 0.35 +0.4 216,020 + Rv0185 0.60 +0.3 216,021 - Rv0185 0.60 +2.5 216,076 + Rv0185 0.71 +0.7 216,076 + Rv0185 0.71 -0.3 216,202 - +1.0 216,378 - -0.1 216,378 - -1.1 216,869 + Rv0186 0.29 +0.6 217,101 + Rv0186 0.40 -0.4
Or see this region's nucleotide sequence