Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0185

Experiment: Urea 10mM (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0183 and Rv0184 are separated by 41 nucleotidesRv0184 and Rv0185 overlap by 4 nucleotidesRv0185 and Rv0186 are separated by 44 nucleotides Rv0183: Rv0183 - Possible lysophospholipase, at 214,088 to 214,927 Rv0183 Rv0184: Rv0184 - Conserved hypothetical protein, at 214,969 to 215,718 Rv0184 Rv0185: Rv0185 - Conserved hypothetical protein, at 215,715 to 216,224 Rv0185 Rv0186: Rv0186 - Probable beta-glucosidase BglS (gentiobiase) (cellobiase) (beta-D-glucoside glucohydrolase), at 216,269 to 218,344 Rv0186 Position (kb) 215 216 217Strain fitness (log2 ratio) -2 -1 0 1 2at 214.744 kb on - strand, within Rv0183at 214.744 kb on - strand, within Rv0183at 215.200 kb on + strand, within Rv0184at 215.200 kb on + strand, within Rv0184at 215.318 kb on + strand, within Rv0184at 215.319 kb on - strand, within Rv0184at 215.319 kb on - strand, within Rv0184at 215.320 kb on + strand, within Rv0184at 215.761 kb on - strandat 215.895 kb on + strand, within Rv0185at 215.895 kb on + strand, within Rv0185at 215.896 kb on - strand, within Rv0185at 216.020 kb on + strand, within Rv0185at 216.021 kb on - strand, within Rv0185at 216.076 kb on + strand, within Rv0185at 216.076 kb on + strand, within Rv0185at 216.202 kb on - strandat 216.378 kb on - strandat 216.378 kb on - strandat 216.869 kb on + strand, within Rv0186at 217.101 kb on + strand, within Rv0186

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Per-strain Table

Position Strand Gene LocusTag Fraction Urea 10mM (N)
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214,744 - Rv0183 0.78 -0.6
214,744 - Rv0183 0.78 -0.3
215,200 + Rv0184 0.31 -0.1
215,200 + Rv0184 0.31 -1.9
215,318 + Rv0184 0.47 -0.1
215,319 - Rv0184 0.47 -0.2
215,319 - Rv0184 0.47 -0.5
215,320 + Rv0184 0.47 -1.9
215,761 - +0.2
215,895 + Rv0185 0.35 +0.7
215,895 + Rv0185 0.35 -0.3
215,896 - Rv0185 0.35 +0.4
216,020 + Rv0185 0.60 +0.3
216,021 - Rv0185 0.60 +2.5
216,076 + Rv0185 0.71 +0.7
216,076 + Rv0185 0.71 -0.3
216,202 - +1.0
216,378 - -0.1
216,378 - -1.1
216,869 + Rv0186 0.29 +0.6
217,101 + Rv0186 0.40 -0.4

Or see this region's nucleotide sequence