Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3371 and Rv3372 are separated by 41 nucleotides Rv3372 and Rv3373 are separated by 236 nucleotides Rv3373 and Rv3374 are separated by 0 nucleotides
Rv3371: Rv3371 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 3,784,932 to 3,786,272
Rv3371
Rv3372: Rv3372 - Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP), at 3,786,314 to 3,787,489
Rv3372
Rv3373: Rv3373 - Probable enoyl-CoA hydratase EchA18 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,787,726 to 3,788,367
Rv3373
Rv3374: Rv3374 - Probable enoyl-CoA hydratase (fragment) EchA18.1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,788,368 to 3,788,616
Rv3374
Position (kb)
3786
3787
3788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3785.858 kb on + strand, within Rv3371 at 3786.085 kb on - strand, within Rv3371 at 3786.085 kb on - strand, within Rv3371 at 3786.179 kb on + strand at 3786.179 kb on + strand at 3787.513 kb on - strand at 3787.513 kb on - strand at 3787.662 kb on + strand at 3787.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 3,785,858 + Rv3371 0.69 -0.4 3,786,085 - Rv3371 0.86 +0.1 3,786,085 - Rv3371 0.86 -0.7 3,786,179 + -0.1 3,786,179 + -2.5 3,787,513 - -0.6 3,787,513 - -0.2 3,787,662 + +1.8 3,787,663 - +0.5
Or see this region's nucleotide sequence