Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3372

Experiment: 7H9 with Cumene hydroperoxide 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3371 and Rv3372 are separated by 41 nucleotidesRv3372 and Rv3373 are separated by 236 nucleotidesRv3373 and Rv3374 are separated by 0 nucleotides Rv3371: Rv3371 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 3,784,932 to 3,786,272 Rv3371 Rv3372: Rv3372 - Trehalose 6-phosphate phosphatase OtsB2 (trehalose-phosphatase) (TPP), at 3,786,314 to 3,787,489 Rv3372 Rv3373: Rv3373 - Probable enoyl-CoA hydratase EchA18 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,787,726 to 3,788,367 Rv3373 Rv3374: Rv3374 - Probable enoyl-CoA hydratase (fragment) EchA18.1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,788,368 to 3,788,616 Rv3374 Position (kb) 3786 3787 3788Strain fitness (log2 ratio) -2 -1 0 1 2at 3785.858 kb on + strand, within Rv3371at 3786.085 kb on - strand, within Rv3371at 3786.085 kb on - strand, within Rv3371at 3786.179 kb on + strandat 3786.179 kb on + strandat 3787.513 kb on - strandat 3787.513 kb on - strandat 3787.662 kb on + strandat 3787.663 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM
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3,785,858 + Rv3371 0.69 -0.4
3,786,085 - Rv3371 0.86 +0.1
3,786,085 - Rv3371 0.86 -0.7
3,786,179 + -0.1
3,786,179 + -2.5
3,787,513 - -0.6
3,787,513 - -0.2
3,787,662 + +1.8
3,787,663 - +0.5

Or see this region's nucleotide sequence