Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3278c and Rv3279c are separated by 42 nucleotides Rv3279c and Rv3280 are separated by 49 nucleotides Rv3280 and Rv3281 overlap by 20 nucleotides Rv3281 and Rv3282 overlap by 4 nucleotides
Rv3278c: Rv3278c - Probable conserved transmembrane protein, at 3,660,651 to 3,661,169
Rv3278c
Rv3279c: Rv3279c - Possible bifunctional protein BirA: biotin operon repressor + biotin--[acetyl-CoA-carboxylase] synthetase (biotin--protein ligase), at 3,661,212 to 3,662,012
Rv3279c
Rv3280: Rv3280 - Probable propionyl-CoA carboxylase beta chain 5 AccD5 (pccase) (propanoyl-CoA:carbon dioxide ligase), at 3,662,062 to 3,663,708
Rv3280
Rv3281: Rv3281 - Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (epsilon chain) AccE5, at 3,663,689 to 3,664,222
Rv3281
Rv3282: Rv3282 - Conserved hypothetical protein, at 3,664,219 to 3,664,887
Rv3282
Position (kb) 3662
3663
3664 Strain fitness (log2 ratio) -1
0
1 at 3661.162 kb on + strand at 3661.181 kb on + strand at 3661.213 kb on + strand at 3662.049 kb on - strand at 3664.564 kb on + strand, within Rv3282 at 3664.678 kb on + strand, within Rv3282
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 3,661,162 + -0.0 3,661,181 + -0.3 3,661,213 + +0.1 3,662,049 - -0.4 3,664,564 + Rv3282 0.52 -0.6 3,664,678 + Rv3282 0.69 +0.5
Or see this region's nucleotide sequence