Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2274c and Rv2274A are separated by 33 nucleotides Rv2274A and Rv2275 overlap by 205 nucleotides Rv2275 and Rv2276 overlap by 4 nucleotides Rv2276 and Rv2277c are separated by 184 nucleotides
Rv2274c: Rv2274c - Possible toxin MazF8, at 2,546,488 to 2,546,805
Rv2274c
Rv2274A: Rv2274A - Possible antitoxin MazE8, at 2,546,839 to 2,547,087
Rv2274A
Rv2275: Rv2275 - Conserved hypothetical protein, at 2,546,883 to 2,547,752
Rv2275
Rv2276: Rv2276 - Cytochrome P450 121 Cyp121, at 2,547,749 to 2,548,939
Rv2276
Rv2277c: Rv2277c - Possible glycerolphosphodiesterase, at 2,549,124 to 2,550,029
Rv2277c
Position (kb)
2547
2548
2549 Strain fitness (log2 ratio)
-1
0
1 at 2546.779 kb on - strand at 2546.800 kb on - strand at 2547.160 kb on - strand, within Rv2275 at 2547.567 kb on + strand, within Rv2275 at 2547.568 kb on - strand, within Rv2275 at 2547.672 kb on + strand at 2548.234 kb on + strand, within Rv2276 at 2548.497 kb on + strand, within Rv2276 at 2549.512 kb on - strand, within Rv2277c at 2549.598 kb on + strand, within Rv2277c at 2549.598 kb on + strand, within Rv2277c at 2549.800 kb on + strand, within Rv2277c at 2549.877 kb on - strand, within Rv2277c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 2,546,779 - -1.0 2,546,800 - -0.2 2,547,160 - Rv2275 0.32 -1.2 2,547,567 + Rv2275 0.79 -0.3 2,547,568 - Rv2275 0.79 -0.7 2,547,672 + -0.6 2,548,234 + Rv2276 0.41 +0.8 2,548,497 + Rv2276 0.63 +0.5 2,549,512 - Rv2277c 0.43 -1.2 2,549,598 + Rv2277c 0.52 -1.1 2,549,598 + Rv2277c 0.52 -0.0 2,549,800 + Rv2277c 0.75 +0.4 2,549,877 - Rv2277c 0.83 +0.1
Or see this region's nucleotide sequence