Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2247

Experiment: 7H9 with Cumene hydroperoxide 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2246 and Rv2247 are separated by 30 nucleotidesRv2247 and Rv2248 are separated by 195 nucleotides Rv2246: Rv2246 - 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I), at 2,519,396 to 2,520,712 Rv2246 Rv2247: Rv2247 - Acetyl/propionyl-CoA carboxylase (beta subunit) AccD6, at 2,520,743 to 2,522,164 Rv2247 Rv2248: Rv2248 - Conserved hypothetical protein, at 2,522,360 to 2,523,175 Rv2248 Position (kb) 2520 2521 2522 2523Strain fitness (log2 ratio) -1 0 1at 2522.162 kb on + strandat 2522.304 kb on + strandat 2522.578 kb on - strand, within Rv2248at 2522.736 kb on + strand, within Rv2248at 2522.827 kb on - strand, within Rv2248

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM
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2,522,162 + -0.9
2,522,304 + -1.6
2,522,578 - Rv2248 0.27 -1.5
2,522,736 + Rv2248 0.46 -0.7
2,522,827 - Rv2248 0.57 -1.2

Or see this region's nucleotide sequence