Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2245

Experiment: 7H9 with Cumene hydroperoxide 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2243 and Rv2244 are separated by 75 nucleotidesRv2244 and Rv2245 overlap by 4 nucleotidesRv2245 and Rv2246 are separated by 30 nucleotides Rv2243: Rv2243 - Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT), at 2,516,787 to 2,517,695 Rv2243 Rv2244: Rv2244 - Meromycolate extension acyl carrier protein AcpM, at 2,517,771 to 2,518,118 Rv2244 Rv2245: Rv2245 - 3-oxoacyl-[acyl-carrier protein] synthase 1 KasA (beta-ketoacyl-ACP synthase) (KAS I), at 2,518,115 to 2,519,365 Rv2245 Rv2246: Rv2246 - 3-oxoacyl-[acyl-carrier protein] synthase 2 KasB (beta-ketoacyl-ACP synthase) (KAS I), at 2,519,396 to 2,520,712 Rv2246 Position (kb) 2518 2519 2520Strain fitness (log2 ratio) -1 0 1at 2517.691 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM
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2,517,691 + +0.2

Or see this region's nucleotide sequence