Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1012

Experiment: 7H9 with Cumene hydroperoxide 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1011 and Rv1012 are separated by 16 nucleotidesRv1012 and Rv1013 are separated by 203 nucleotides Rv1011: Rv1011 - Probable 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase IspE (CMK) (4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase), at 1,130,191 to 1,131,111 Rv1011 Rv1012: Rv1012 - Hypothetical protein, at 1,131,128 to 1,131,421 Rv1012 Rv1013: Rv1013 - Putative polyketide synthase Pks16, at 1,131,625 to 1,133,259 Rv1013 Position (kb) 1131 1132Strain fitness (log2 ratio) -2 -1 0 1at 1130.143 kb on + strandat 1130.144 kb on - strandat 1131.269 kb on + strand, within Rv1012at 1131.269 kb on + strand, within Rv1012at 1131.270 kb on - strand, within Rv1012at 1131.328 kb on + strand, within Rv1012at 1131.460 kb on - strandat 1131.460 kb on - strandat 1132.024 kb on - strand, within Rv1013at 1132.024 kb on - strand, within Rv1013at 1132.024 kb on - strand, within Rv1013at 1132.156 kb on + strand, within Rv1013at 1132.157 kb on - strand, within Rv1013at 1132.192 kb on + strand, within Rv1013at 1132.192 kb on + strand, within Rv1013at 1132.357 kb on + strand, within Rv1013at 1132.357 kb on + strand, within Rv1013at 1132.358 kb on - strand, within Rv1013at 1132.358 kb on - strand, within Rv1013

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM
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1,130,143 + -0.0
1,130,144 - -0.5
1,131,269 + Rv1012 0.48 -0.1
1,131,269 + Rv1012 0.48 +1.2
1,131,270 - Rv1012 0.48 +0.2
1,131,328 + Rv1012 0.68 -0.4
1,131,460 - -1.2
1,131,460 - -0.8
1,132,024 - Rv1013 0.24 -2.3
1,132,024 - Rv1013 0.24 -1.9
1,132,024 - Rv1013 0.24 -0.8
1,132,156 + Rv1013 0.32 -1.3
1,132,157 - Rv1013 0.33 -1.3
1,132,192 + Rv1013 0.35 -1.7
1,132,192 + Rv1013 0.35 +0.0
1,132,357 + Rv1013 0.45 -0.9
1,132,357 + Rv1013 0.45 -2.2
1,132,358 - Rv1013 0.45 -1.7
1,132,358 - Rv1013 0.45 -2.1

Or see this region's nucleotide sequence