Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0634B and Rv0635 are separated by 50 nucleotides Rv0635 and Rv0636 overlap by 14 nucleotides Rv0636 and Rv0637 are separated by 3 nucleotides Rv0637 and GFF666 are separated by 198 nucleotides GFF666 and Rv0638 are separated by 140 nucleotides Rv0638 and Rv0639 are separated by 31 nucleotides
Rv0634B: Rv0634B - 50S ribosomal protein L33 RpmG2, at 731,712 to 731,879
Rv0634B
Rv0635: Rv0635 - (3R)-hydroxyacyl-ACP dehydratase subunit HadA, at 731,930 to 732,406
Rv0635
Rv0636: Rv0636 - (3R)-hydroxyacyl-ACP dehydratase subunit HadB, at 732,393 to 732,821
Rv0636
Rv0637: Rv0637 - (3R)-hydroxyacyl-ACP dehydratase subunit HadC, at 732,825 to 733,325
Rv0637
GFF666 - tRNA-Trp, at 733,524 to 733,596
GFF666
Rv0638: Rv0638 - Probable preprotein translocase SecE1, at 733,737 to 734,222
Rv0638
Rv0639: Rv0639 - Probable transcription antitermination protein NusG, at 734,254 to 734,970
Rv0639
Position (kb) 732
733
734 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 731.912 kb on + strand at 731.912 kb on + strand at 731.916 kb on + strand at 731.916 kb on + strand at 733.324 kb on - strand at 733.327 kb on + strand at 733.328 kb on - strand at 733.352 kb on - strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.652 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.656 kb on + strand at 733.716 kb on + strand at 733.716 kb on + strand at 733.716 kb on + strand at 733.719 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 731,912 + -0.1 731,912 + +0.0 731,916 + -0.4 731,916 + -0.7 733,324 - -0.3 733,327 + -1.1 733,328 - -1.1 733,352 - +2.2 733,652 + -1.0 733,652 + -0.1 733,652 + -0.8 733,652 + +0.7 733,652 + +0.7 733,652 + +0.0 733,652 + -0.3 733,652 + -0.0 733,656 + -0.7 733,656 + +1.0 733,656 + -0.6 733,656 + +0.2 733,656 + +0.4 733,656 + -0.4 733,656 + -3.3 733,656 + +0.2 733,656 + +0.6 733,716 + -0.6 733,716 + -0.1 733,716 + -0.7 733,719 + -0.3
Or see this region's nucleotide sequence