Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0634c and Rv0634A are separated by 79 nucleotides Rv0634A and GFF660 are separated by 129 nucleotides GFF660 and GFF661 are separated by 36 nucleotides GFF661 and Rv0634B are separated by 35 nucleotides Rv0634B and Rv0635 are separated by 50 nucleotides Rv0635 and Rv0636 overlap by 14 nucleotides Rv0636 and Rv0637 are separated by 3 nucleotides
Rv0634c: Rv0634c - Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II), at 730,320 to 731,033
Rv0634c
Rv0634A: Rv0634A - Unknown protein, at 731,113 to 731,364
Rv0634A
GFF660 - tRNA-Thr, at 731,494 to 731,566
GFF660
GFF661 - tRNA-Met, at 731,603 to 731,676
GFF661
Rv0634B: Rv0634B - 50S ribosomal protein L33 RpmG2, at 731,712 to 731,879
Rv0634B
Rv0635: Rv0635 - (3R)-hydroxyacyl-ACP dehydratase subunit HadA, at 731,930 to 732,406
Rv0635
Rv0636: Rv0636 - (3R)-hydroxyacyl-ACP dehydratase subunit HadB, at 732,393 to 732,821
Rv0636
Rv0637: Rv0637 - (3R)-hydroxyacyl-ACP dehydratase subunit HadC, at 732,825 to 733,325
Rv0637
Position (kb) 731
732
733 Strain fitness (log2 ratio) -1
0
1
2 at 731.014 kb on + strand at 731.015 kb on - strand at 731.015 kb on - strand at 731.044 kb on + strand at 731.044 kb on + strand at 731.045 kb on - strand at 731.101 kb on + strand at 731.101 kb on + strand at 731.102 kb on - strand at 731.102 kb on - strand at 731.171 kb on + strand, within Rv0634A at 731.305 kb on + strand, within Rv0634A at 731.305 kb on + strand, within Rv0634A at 731.306 kb on - strand, within Rv0634A at 731.394 kb on + strand at 731.394 kb on + strand at 731.912 kb on + strand at 731.912 kb on + strand at 731.916 kb on + strand at 731.916 kb on + strand at 733.324 kb on - strand at 733.327 kb on + strand at 733.328 kb on - strand at 733.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 731,014 + -0.1 731,015 - -0.9 731,015 - -0.6 731,044 + -0.8 731,044 + -0.1 731,045 - -0.8 731,101 + +0.0 731,101 + -0.2 731,102 - -0.2 731,102 - -0.7 731,171 + Rv0634A 0.23 -0.4 731,305 + Rv0634A 0.76 -1.7 731,305 + Rv0634A 0.76 -0.4 731,306 - Rv0634A 0.77 -0.7 731,394 + -1.1 731,394 + -0.2 731,912 + -0.1 731,912 + +0.0 731,916 + -0.4 731,916 + -0.7 733,324 - -0.3 733,327 + -1.1 733,328 - -1.1 733,352 - +2.2
Or see this region's nucleotide sequence