Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0614 and Rv0615 overlap by 4 nucleotides Rv0615 and Rv0616c overlap by 4 nucleotides Rv0616c and Rv0616A overlap by 69 nucleotides Rv0616A and Rv0617 overlap by 4 nucleotides Rv0617 and Rv0618 are separated by 128 nucleotides Rv0618 and Rv0619 overlap by 58 nucleotides
Rv0614: Rv0614 - Conserved hypothetical protein, at 709,356 to 710,348
Rv0614
Rv0615: Rv0615 - Probable integral membrane protein, at 710,345 to 710,587
Rv0615
Rv0616c: Rv0616c - Hypothetical protein, at 710,584 to 710,850
Rv0616c
Rv0616A: Rv0616A - Possible antitoxin VapB29, at 710,782 to 711,009
Rv0616A
Rv0617: Rv0617 - Possible toxin VapC29. Contains PIN domain., at 711,006 to 711,407
Rv0617
Rv0618: Rv0618 - Probable galactose-1-phosphate uridylyltransferase GalTa [first part], at 711,536 to 712,231
Rv0618
Rv0619: Rv0619 - Probable galactose-1-phosphate uridylyltransferase GalTb [second part], at 712,174 to 712,719
Rv0619
Position (kb) 711
712 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 710.028 kb on - strand, within Rv0614 at 710.071 kb on + strand, within Rv0614 at 710.072 kb on - strand, within Rv0614 at 710.157 kb on + strand, within Rv0614 at 710.323 kb on + strand at 710.323 kb on + strand at 710.355 kb on + strand at 710.355 kb on + strand at 710.435 kb on + strand, within Rv0615 at 710.436 kb on - strand, within Rv0615 at 710.586 kb on - strand at 710.790 kb on + strand at 710.790 kb on + strand at 710.993 kb on + strand at 711.300 kb on + strand, within Rv0617 at 711.301 kb on - strand, within Rv0617 at 711.301 kb on - strand, within Rv0617 at 711.470 kb on + strand at 711.476 kb on + strand at 711.477 kb on - strand at 711.477 kb on - strand at 711.477 kb on - strand at 711.905 kb on + strand, within Rv0618 at 712.214 kb on - strand at 712.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 710,028 - Rv0614 0.68 -0.3 710,071 + Rv0614 0.72 +2.0 710,072 - Rv0614 0.72 +0.2 710,157 + Rv0614 0.81 +0.0 710,323 + -0.7 710,323 + -0.9 710,355 + -0.1 710,355 + -0.5 710,435 + Rv0615 0.37 +0.6 710,436 - Rv0615 0.37 +0.3 710,586 - +0.1 710,790 + +2.0 710,790 + +1.0 710,993 + -0.4 711,300 + Rv0617 0.73 +0.1 711,301 - Rv0617 0.73 +0.4 711,301 - Rv0617 0.73 +0.8 711,470 + -0.5 711,476 + +0.6 711,477 - +0.9 711,477 - +0.1 711,477 - -2.3 711,905 + Rv0618 0.53 +5.0 712,214 - -1.4 712,225 + -1.0
Or see this region's nucleotide sequence