Experiment: 7H9 with Cumene hydroperoxide 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0494 and Rv0495c are separated by 0 nucleotides Rv0495c and Rv0496 are separated by 79 nucleotides Rv0496 and Rv0497 overlap by 4 nucleotides Rv0497 and Rv0498 are separated by 15 nucleotides
Rv0494: Rv0494 - Probable transcriptional regulatory protein (probably GntR-family), at 584,695 to 585,423
Rv0494
Rv0495c: Rv0495c - Conserved hypothetical protein, at 585,424 to 586,314
Rv0495c
Rv0496: Rv0496 - Conserved hypothetical protein, at 586,394 to 587,380
Rv0496
Rv0497: Rv0497 - Probable conserved transmembrane protein, at 587,377 to 588,309
Rv0497
Rv0498: Rv0498 - Conserved hypothetical protein, at 588,325 to 589,167
Rv0498
Position (kb) 586
587
588 Strain fitness (log2 ratio) -1
0
1 at 585.422 kb on - strand at 585.422 kb on - strand at 585.422 kb on - strand at 585.426 kb on - strand at 585.426 kb on - strand at 587.001 kb on + strand, within Rv0496 at 587.049 kb on + strand, within Rv0496 at 587.084 kb on - strand, within Rv0496 at 587.084 kb on - strand, within Rv0496 at 587.090 kb on - strand, within Rv0496 at 587.199 kb on + strand, within Rv0496 at 587.200 kb on - strand, within Rv0496 at 588.155 kb on + strand, within Rv0497 at 588.155 kb on + strand, within Rv0497 at 588.164 kb on + strand, within Rv0497 at 588.170 kb on + strand, within Rv0497
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Cumene hydroperoxide 0.05 mM remove 585,422 - +0.2 585,422 - -0.7 585,422 - -0.3 585,426 - -1.2 585,426 - -1.2 587,001 + Rv0496 0.61 +1.6 587,049 + Rv0496 0.66 +1.0 587,084 - Rv0496 0.70 +0.1 587,084 - Rv0496 0.70 -0.4 587,090 - Rv0496 0.71 +0.4 587,199 + Rv0496 0.82 -0.1 587,200 - Rv0496 0.82 -0.5 588,155 + Rv0497 0.83 -0.5 588,155 + Rv0497 0.83 -1.5 588,164 + Rv0497 0.84 -0.8 588,170 + Rv0497 0.85 -1.3
Or see this region's nucleotide sequence