Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0325

Experiment: 7H9 with Sutezolid 0.12 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0323c and Rv0324 are separated by 100 nucleotidesRv0324 and Rv0325 are separated by 6 nucleotidesRv0325 and Rv0326 are separated by 9 nucleotidesRv0326 and Rv0327c overlap by 33 nucleotides Rv0323c: Rv0323c - Conserved hypothetical protein, at 390,580 to 391,251 Rv0323c Rv0324: Rv0324 - Possible transcriptional regulatory protein (possibly ArsR-family), at 391,352 to 392,032 Rv0324 Rv0325: Rv0325 - Hypothetical protein, at 392,039 to 392,263 Rv0325 Rv0326: Rv0326 - Hypothetical protein, at 392,273 to 392,728 Rv0326 Rv0327c: Rv0327c - Possible cytochrome P450 135A1 Cyp135A1, at 392,696 to 394,045 Rv0327c Position (kb) 392 393Strain fitness (log2 ratio) -2 -1 0 1at 391.303 kb on + strandat 391.584 kb on - strand, within Rv0324at 391.795 kb on + strand, within Rv0324at 391.796 kb on - strand, within Rv0324at 391.877 kb on + strand, within Rv0324at 391.892 kb on + strand, within Rv0324at 392.058 kb on + strandat 392.059 kb on - strandat 392.059 kb on - strandat 392.141 kb on + strand, within Rv0325at 392.141 kb on + strand, within Rv0325at 392.169 kb on + strand, within Rv0325at 392.169 kb on + strand, within Rv0325at 392.170 kb on - strand, within Rv0325at 392.170 kb on - strand, within Rv0325at 392.170 kb on - strand, within Rv0325at 392.170 kb on - strand, within Rv0325at 392.171 kb on + strand, within Rv0325at 392.172 kb on - strand, within Rv0325at 392.172 kb on - strand, within Rv0325at 392.172 kb on - strand, within Rv0325at 392.172 kb on - strand, within Rv0325at 392.250 kb on - strandat 393.182 kb on + strand, within Rv0327c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Sutezolid 0.12 ug/mL
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391,303 + -2.3
391,584 - Rv0324 0.34 +1.0
391,795 + Rv0324 0.65 -0.4
391,796 - Rv0324 0.65 +0.0
391,877 + Rv0324 0.77 -1.7
391,892 + Rv0324 0.79 +0.2
392,058 + +0.4
392,059 - +0.2
392,059 - +1.3
392,141 + Rv0325 0.45 +0.2
392,141 + Rv0325 0.45 -0.1
392,169 + Rv0325 0.58 -0.0
392,169 + Rv0325 0.58 +0.4
392,170 - Rv0325 0.58 -0.8
392,170 - Rv0325 0.58 -0.3
392,170 - Rv0325 0.58 +0.8
392,170 - Rv0325 0.58 +0.5
392,171 + Rv0325 0.59 +1.7
392,172 - Rv0325 0.59 -0.1
392,172 - Rv0325 0.59 -0.4
392,172 - Rv0325 0.59 -0.4
392,172 - Rv0325 0.59 +1.4
392,250 - -0.6
393,182 + Rv0327c 0.36 +0.1

Or see this region's nucleotide sequence