Experiment: 7H9 with Amikacin di2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3175 and Rv3176c overlap by 4 nucleotides Rv3176c and Rv3177 are separated by 146 nucleotides
Rv3175: Rv3175 - Possible amidase (aminohydrolase), at 3,542,860 to 3,544,347
Rv3175
Rv3176c: Rv3176c - Probable epoxide hydrolase MesT (epoxide hydratase) (arene-oxide hydratase), at 3,544,344 to 3,545,300
Rv3176c
Rv3177: Rv3177 - Possible peroxidase (non-haem peroxidase), at 3,545,447 to 3,546,307
Rv3177
Position (kb)
3544
3545
3546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3543.395 kb on - strand, within Rv3175 at 3543.635 kb on - strand, within Rv3175 at 3543.963 kb on - strand, within Rv3175 at 3544.151 kb on - strand, within Rv3175 at 3544.230 kb on + strand at 3544.289 kb on + strand at 3544.289 kb on + strand at 3544.290 kb on - strand at 3544.346 kb on - strand at 3544.346 kb on - strand at 3544.371 kb on - strand at 3544.927 kb on + strand, within Rv3176c at 3545.017 kb on + strand, within Rv3176c at 3545.054 kb on - strand, within Rv3176c at 3545.063 kb on - strand, within Rv3176c at 3545.119 kb on + strand, within Rv3176c at 3545.119 kb on + strand, within Rv3176c at 3545.181 kb on + strand, within Rv3176c at 3545.181 kb on + strand, within Rv3176c at 3545.181 kb on + strand, within Rv3176c at 3545.392 kb on + strand at 3545.401 kb on + strand at 3545.401 kb on + strand at 3545.484 kb on - strand at 3545.534 kb on + strand, within Rv3177
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Amikacin di2 ug/mL remove 3,543,395 - Rv3175 0.36 +0.1 3,543,635 - Rv3175 0.52 -0.1 3,543,963 - Rv3175 0.74 +0.2 3,544,151 - Rv3175 0.87 -0.9 3,544,230 + -0.4 3,544,289 + -0.6 3,544,289 + +0.7 3,544,290 - -0.0 3,544,346 - -0.1 3,544,346 - +2.9 3,544,371 - +0.3 3,544,927 + Rv3176c 0.61 -1.5 3,545,017 + Rv3176c 0.70 -0.7 3,545,054 - Rv3176c 0.74 -2.9 3,545,063 - Rv3176c 0.75 -0.5 3,545,119 + Rv3176c 0.81 -0.7 3,545,119 + Rv3176c 0.81 -1.7 3,545,181 + Rv3176c 0.87 -0.7 3,545,181 + Rv3176c 0.87 -0.4 3,545,181 + Rv3176c 0.87 +1.9 3,545,392 + -0.3 3,545,401 + -0.4 3,545,401 + -0.1 3,545,484 - -0.1 3,545,534 + Rv3177 0.10 -0.4
Or see this region's nucleotide sequence