Experiment: 7H9 with Amikacin di2 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0486 and Rv0487 overlap by 4 nucleotides Rv0487 and Rv0488 are separated by 325 nucleotides Rv0488 and Rv0489 are separated by 156 nucleotides
Rv0486: Rv0486 - Glycosyltransferase MshA, at 575,348 to 576,790
Rv0486
Rv0487: Rv0487 - Conserved hypothetical protein, at 576,787 to 577,338
Rv0487
Rv0488: Rv0488 - Probable conserved integral membrane protein, at 577,664 to 578,269
Rv0488
Rv0489: Rv0489 - Probable phosphoglycerate mutase 1 Gpm1 (phosphoglyceromutase) (PGAM) (BPG-dependent PGAM), at 578,426 to 579,175
Rv0489
Position (kb)
577
578
579 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 576.689 kb on + strand at 576.853 kb on + strand, within Rv0487 at 576.853 kb on + strand, within Rv0487 at 577.013 kb on + strand, within Rv0487 at 577.013 kb on + strand, within Rv0487 at 577.013 kb on + strand, within Rv0487 at 577.014 kb on - strand, within Rv0487 at 577.014 kb on - strand, within Rv0487 at 577.014 kb on - strand, within Rv0487 at 577.060 kb on + strand, within Rv0487 at 577.087 kb on + strand, within Rv0487 at 577.088 kb on - strand, within Rv0487 at 577.157 kb on + strand, within Rv0487 at 577.158 kb on - strand, within Rv0487 at 577.158 kb on - strand, within Rv0487 at 577.284 kb on - strand at 577.337 kb on - strand at 577.337 kb on - strand at 577.337 kb on - strand at 577.337 kb on - strand at 577.337 kb on - strand at 577.337 kb on - strand at 577.337 kb on - strand at 577.498 kb on + strand at 577.499 kb on - strand at 577.499 kb on - strand at 577.619 kb on + strand at 577.734 kb on - strand, within Rv0488 at 577.734 kb on - strand, within Rv0488 at 577.832 kb on - strand, within Rv0488 at 578.003 kb on + strand, within Rv0488 at 578.003 kb on + strand, within Rv0488 at 578.098 kb on - strand, within Rv0488 at 578.098 kb on - strand, within Rv0488 at 578.098 kb on - strand, within Rv0488 at 578.278 kb on + strand at 578.291 kb on + strand at 578.291 kb on + strand at 579.173 kb on + strand at 579.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Amikacin di2 ug/mL remove 576,689 + -0.1 576,853 + Rv0487 0.12 -0.2 576,853 + Rv0487 0.12 +1.0 577,013 + Rv0487 0.41 -0.2 577,013 + Rv0487 0.41 -0.7 577,013 + Rv0487 0.41 +0.1 577,014 - Rv0487 0.41 +0.7 577,014 - Rv0487 0.41 -1.7 577,014 - Rv0487 0.41 +1.1 577,060 + Rv0487 0.49 -0.6 577,087 + Rv0487 0.54 -0.5 577,088 - Rv0487 0.55 -0.2 577,157 + Rv0487 0.67 +0.9 577,158 - Rv0487 0.67 -0.7 577,158 - Rv0487 0.67 +0.3 577,284 - +0.3 577,337 - +0.3 577,337 - -2.4 577,337 - -1.1 577,337 - -0.3 577,337 - -0.5 577,337 - -1.1 577,337 - +0.3 577,498 + -0.7 577,499 - -0.4 577,499 - -0.2 577,619 + -0.2 577,734 - Rv0488 0.12 +0.9 577,734 - Rv0488 0.12 -1.1 577,832 - Rv0488 0.28 -0.4 578,003 + Rv0488 0.56 +1.9 578,003 + Rv0488 0.56 +0.7 578,098 - Rv0488 0.72 -0.2 578,098 - Rv0488 0.72 -0.3 578,098 - Rv0488 0.72 -0.6 578,278 + -0.5 578,291 + -0.6 578,291 + +0.2 579,173 + -0.7 579,174 - -0.1
Or see this region's nucleotide sequence