Experiment: M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04103 and MPMX20_04104 are separated by 14 nucleotides MPMX20_04104 and MPMX20_04105 overlap by 4 nucleotides MPMX20_04105 and MPMX20_04106 overlap by 32 nucleotides MPMX20_04106 and MPMX20_04107 are separated by 6 nucleotides
MPMX20_04103: MPMX20_04103 - Arabinose 5-phosphate isomerase KdsD, at 4,297,405 to 4,298,391
_04103
MPMX20_04104: MPMX20_04104 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC, at 4,298,406 to 4,298,972
_04104
MPMX20_04105: MPMX20_04105 - Lipopolysaccharide export system protein LptC, at 4,298,969 to 4,299,544
_04105
MPMX20_04106: MPMX20_04106 - Lipopolysaccharide export system protein LptA, at 4,299,513 to 4,300,073
_04106
MPMX20_04107: MPMX20_04107 - Lipopolysaccharide export system ATP-binding protein LptB, at 4,300,080 to 4,300,805
_04107
Position (kb)
4298
4299
4300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4298.008 kb on - strand, within MPMX20_04103 at 4298.014 kb on - strand, within MPMX20_04103 at 4298.019 kb on + strand, within MPMX20_04103 at 4298.037 kb on - strand, within MPMX20_04103 at 4298.039 kb on + strand, within MPMX20_04103 at 4298.049 kb on - strand, within MPMX20_04103 at 4298.198 kb on + strand, within MPMX20_04103 at 4298.198 kb on + strand, within MPMX20_04103 at 4298.217 kb on + strand, within MPMX20_04103 at 4298.217 kb on + strand, within MPMX20_04103 at 4298.245 kb on - strand, within MPMX20_04103 at 4298.262 kb on + strand, within MPMX20_04103 at 4298.320 kb on - strand at 4298.348 kb on + strand at 4298.380 kb on + strand at 4298.380 kb on + strand at 4298.380 kb on + strand at 4298.386 kb on + strand at 4298.396 kb on + strand at 4298.396 kb on + strand at 4298.396 kb on + strand at 4298.437 kb on + strand at 4298.437 kb on + strand at 4298.452 kb on + strand at 4298.459 kb on + strand at 4298.494 kb on + strand, within MPMX20_04104 at 4298.605 kb on + strand, within MPMX20_04104 at 4298.680 kb on + strand, within MPMX20_04104 at 4298.819 kb on + strand, within MPMX20_04104 at 4298.821 kb on + strand, within MPMX20_04104 at 4298.869 kb on + strand, within MPMX20_04104 at 4298.951 kb on + strand at 4299.336 kb on + strand, within MPMX20_04105 at 4299.373 kb on + strand, within MPMX20_04105 at 4299.373 kb on + strand, within MPMX20_04105 at 4299.407 kb on + strand, within MPMX20_04105 at 4299.447 kb on + strand, within MPMX20_04105 at 4299.499 kb on + strand at 4300.022 kb on + strand at 4300.022 kb on + strand at 4300.022 kb on + strand at 4300.040 kb on + strand at 4300.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031 remove 4,298,008 - MPMX20_04103 0.61 +2.6 4,298,014 - MPMX20_04103 0.62 -1.9 4,298,019 + MPMX20_04103 0.62 +0.2 4,298,037 - MPMX20_04103 0.64 -0.2 4,298,039 + MPMX20_04103 0.64 -0.7 4,298,049 - MPMX20_04103 0.65 -0.2 4,298,198 + MPMX20_04103 0.80 -0.4 4,298,198 + MPMX20_04103 0.80 -1.5 4,298,217 + MPMX20_04103 0.82 +0.2 4,298,217 + MPMX20_04103 0.82 -1.7 4,298,245 - MPMX20_04103 0.85 +0.4 4,298,262 + MPMX20_04103 0.87 -1.0 4,298,320 - +0.8 4,298,348 + -1.0 4,298,380 + +1.0 4,298,380 + -0.9 4,298,380 + -2.4 4,298,386 + -3.3 4,298,396 + -1.5 4,298,396 + +0.3 4,298,396 + +0.2 4,298,437 + -1.0 4,298,437 + +2.0 4,298,452 + -0.8 4,298,459 + +0.0 4,298,494 + MPMX20_04104 0.16 -0.3 4,298,605 + MPMX20_04104 0.35 +0.6 4,298,680 + MPMX20_04104 0.48 -0.5 4,298,819 + MPMX20_04104 0.73 -0.2 4,298,821 + MPMX20_04104 0.73 -2.0 4,298,869 + MPMX20_04104 0.82 -0.8 4,298,951 + +0.0 4,299,336 + MPMX20_04105 0.64 -1.2 4,299,373 + MPMX20_04105 0.70 +0.6 4,299,373 + MPMX20_04105 0.70 -1.9 4,299,407 + MPMX20_04105 0.76 -0.4 4,299,447 + MPMX20_04105 0.83 -2.9 4,299,499 + -1.0 4,300,022 + -0.2 4,300,022 + +0.3 4,300,022 + +0.5 4,300,040 + -0.7 4,300,058 + -2.7
Or see this region's nucleotide sequence