Experiment: M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02632 and MPMX20_02633 are separated by 36 nucleotides MPMX20_02633 and MPMX20_02634 are separated by 3 nucleotides MPMX20_02634 and MPMX20_02635 overlap by 4 nucleotides MPMX20_02635 and MPMX20_02636 overlap by 1 nucleotides
MPMX20_02632: MPMX20_02632 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,776,390 to 2,777,244
_02632
MPMX20_02633: MPMX20_02633 - hypothetical protein, at 2,777,281 to 2,778,090
_02633
MPMX20_02634: MPMX20_02634 - Protein YchQ, at 2,778,094 to 2,778,486
_02634
MPMX20_02635: MPMX20_02635 - Release factor glutamine methyltransferase, at 2,778,483 to 2,779,313
_02635
MPMX20_02636: MPMX20_02636 - Peptide chain release factor RF1, at 2,779,313 to 2,780,395
_02636
Position (kb)
2778
2779 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2777.279 kb on + strand at 2777.287 kb on - strand at 2777.320 kb on - strand at 2777.320 kb on - strand at 2777.320 kb on - strand at 2777.396 kb on - strand, within MPMX20_02633 at 2777.414 kb on + strand, within MPMX20_02633 at 2777.464 kb on - strand, within MPMX20_02633 at 2777.637 kb on - strand, within MPMX20_02633 at 2777.654 kb on - strand, within MPMX20_02633 at 2777.654 kb on - strand, within MPMX20_02633 at 2777.776 kb on - strand, within MPMX20_02633 at 2777.811 kb on - strand, within MPMX20_02633 at 2777.848 kb on - strand, within MPMX20_02633 at 2777.982 kb on - strand, within MPMX20_02633 at 2778.093 kb on - strand at 2778.100 kb on - strand at 2778.111 kb on + strand at 2778.147 kb on + strand, within MPMX20_02634 at 2778.264 kb on + strand, within MPMX20_02634 at 2778.336 kb on - strand, within MPMX20_02634 at 2778.339 kb on + strand, within MPMX20_02634 at 2778.347 kb on - strand, within MPMX20_02634 at 2778.347 kb on - strand, within MPMX20_02634 at 2778.347 kb on - strand, within MPMX20_02634 at 2778.438 kb on + strand, within MPMX20_02634 at 2778.446 kb on + strand, within MPMX20_02634 at 2778.446 kb on + strand, within MPMX20_02634 at 2778.459 kb on - strand at 2778.803 kb on + strand, within MPMX20_02635 at 2778.833 kb on + strand, within MPMX20_02635 at 2778.841 kb on - strand, within MPMX20_02635 at 2778.856 kb on + strand, within MPMX20_02635 at 2778.930 kb on + strand, within MPMX20_02635 at 2779.073 kb on - strand, within MPMX20_02635 at 2779.086 kb on - strand, within MPMX20_02635 at 2779.289 kb on + strand at 2779.297 kb on - strand at 2779.297 kb on - strand at 2779.297 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031 remove 2,777,279 + -0.5 2,777,287 - +0.4 2,777,320 - -0.9 2,777,320 - -1.0 2,777,320 - -2.0 2,777,396 - MPMX20_02633 0.14 -1.5 2,777,414 + MPMX20_02633 0.16 -1.6 2,777,464 - MPMX20_02633 0.23 -1.4 2,777,637 - MPMX20_02633 0.44 -1.5 2,777,654 - MPMX20_02633 0.46 -0.9 2,777,654 - MPMX20_02633 0.46 +0.3 2,777,776 - MPMX20_02633 0.61 +0.1 2,777,811 - MPMX20_02633 0.65 -2.1 2,777,848 - MPMX20_02633 0.70 -2.1 2,777,982 - MPMX20_02633 0.87 -1.1 2,778,093 - -1.0 2,778,100 - -4.8 2,778,111 + -0.2 2,778,147 + MPMX20_02634 0.13 -0.9 2,778,264 + MPMX20_02634 0.43 -0.2 2,778,336 - MPMX20_02634 0.62 -0.0 2,778,339 + MPMX20_02634 0.62 -0.1 2,778,347 - MPMX20_02634 0.64 -0.1 2,778,347 - MPMX20_02634 0.64 -1.2 2,778,347 - MPMX20_02634 0.64 -1.8 2,778,438 + MPMX20_02634 0.88 -0.9 2,778,446 + MPMX20_02634 0.90 +0.3 2,778,446 + MPMX20_02634 0.90 +0.8 2,778,459 - +0.9 2,778,803 + MPMX20_02635 0.39 +0.6 2,778,833 + MPMX20_02635 0.42 -1.5 2,778,841 - MPMX20_02635 0.43 -1.1 2,778,856 + MPMX20_02635 0.45 +0.6 2,778,930 + MPMX20_02635 0.54 +1.9 2,779,073 - MPMX20_02635 0.71 +1.3 2,779,086 - MPMX20_02635 0.73 +0.3 2,779,289 + +1.9 2,779,297 - +1.9 2,779,297 - -0.8 2,779,297 - -0.1
Or see this region's nucleotide sequence