Experiment: M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01612 and MPMX20_01613 are separated by 59 nucleotides MPMX20_01613 and MPMX20_01614 are separated by 80 nucleotides MPMX20_01614 and MPMX20_01615 are separated by 25 nucleotides
MPMX20_01612: MPMX20_01612 - DNA helicase IV, at 1,704,798 to 1,706,852
_01612
MPMX20_01613: MPMX20_01613 - Methylglyoxal synthase, at 1,706,912 to 1,707,370
_01613
MPMX20_01614: MPMX20_01614 - hypothetical protein, at 1,707,451 to 1,708,260
_01614
MPMX20_01615: MPMX20_01615 - putative protein YccU, at 1,708,286 to 1,708,702
_01615
Position (kb)
1706
1707
1708 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1705.961 kb on + strand, within MPMX20_01612 at 1705.968 kb on - strand, within MPMX20_01612 at 1706.012 kb on + strand, within MPMX20_01612 at 1706.035 kb on + strand, within MPMX20_01612 at 1706.159 kb on - strand, within MPMX20_01612 at 1706.227 kb on - strand, within MPMX20_01612 at 1706.263 kb on + strand, within MPMX20_01612 at 1706.351 kb on - strand, within MPMX20_01612 at 1706.392 kb on - strand, within MPMX20_01612 at 1706.440 kb on - strand, within MPMX20_01612 at 1706.518 kb on - strand, within MPMX20_01612 at 1706.623 kb on + strand, within MPMX20_01612 at 1706.623 kb on + strand, within MPMX20_01612 at 1706.750 kb on - strand at 1706.750 kb on - strand at 1706.829 kb on - strand at 1706.855 kb on + strand at 1706.972 kb on + strand, within MPMX20_01613 at 1706.980 kb on - strand, within MPMX20_01613 at 1707.040 kb on - strand, within MPMX20_01613 at 1707.053 kb on + strand, within MPMX20_01613 at 1707.053 kb on + strand, within MPMX20_01613 at 1707.053 kb on + strand, within MPMX20_01613 at 1707.067 kb on + strand, within MPMX20_01613 at 1707.067 kb on - strand, within MPMX20_01613 at 1707.194 kb on + strand, within MPMX20_01613 at 1707.194 kb on - strand, within MPMX20_01613 at 1707.220 kb on + strand, within MPMX20_01613 at 1707.220 kb on + strand, within MPMX20_01613 at 1707.239 kb on + strand, within MPMX20_01613 at 1707.293 kb on - strand, within MPMX20_01613 at 1707.329 kb on - strand at 1707.385 kb on + strand at 1707.393 kb on - strand at 1707.468 kb on + strand at 1707.476 kb on - strand at 1707.602 kb on - strand, within MPMX20_01614 at 1707.628 kb on + strand, within MPMX20_01614 at 1707.643 kb on + strand, within MPMX20_01614 at 1707.709 kb on - strand, within MPMX20_01614 at 1707.712 kb on + strand, within MPMX20_01614 at 1707.770 kb on - strand, within MPMX20_01614 at 1707.779 kb on + strand, within MPMX20_01614 at 1707.827 kb on + strand, within MPMX20_01614 at 1707.827 kb on + strand, within MPMX20_01614 at 1707.835 kb on - strand, within MPMX20_01614 at 1707.851 kb on + strand, within MPMX20_01614 at 1707.878 kb on + strand, within MPMX20_01614 at 1707.902 kb on - strand, within MPMX20_01614 at 1707.951 kb on - strand, within MPMX20_01614 at 1708.041 kb on + strand, within MPMX20_01614 at 1708.191 kb on - strand at 1708.210 kb on - strand at 1708.255 kb on - strand at 1708.302 kb on - strand at 1708.347 kb on - strand, within MPMX20_01615
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031 remove 1,705,961 + MPMX20_01612 0.57 -0.6 1,705,968 - MPMX20_01612 0.57 +0.3 1,706,012 + MPMX20_01612 0.59 -0.7 1,706,035 + MPMX20_01612 0.60 -1.5 1,706,159 - MPMX20_01612 0.66 +0.3 1,706,227 - MPMX20_01612 0.70 -0.5 1,706,263 + MPMX20_01612 0.71 +1.3 1,706,351 - MPMX20_01612 0.76 -0.5 1,706,392 - MPMX20_01612 0.78 -1.4 1,706,440 - MPMX20_01612 0.80 -0.7 1,706,518 - MPMX20_01612 0.84 -0.2 1,706,623 + MPMX20_01612 0.89 +1.1 1,706,623 + MPMX20_01612 0.89 -0.3 1,706,750 - +2.8 1,706,750 - +0.6 1,706,829 - -0.3 1,706,855 + +1.0 1,706,972 + MPMX20_01613 0.13 -0.3 1,706,980 - MPMX20_01613 0.15 -0.2 1,707,040 - MPMX20_01613 0.28 +0.6 1,707,053 + MPMX20_01613 0.31 -0.9 1,707,053 + MPMX20_01613 0.31 -0.6 1,707,053 + MPMX20_01613 0.31 +1.8 1,707,067 + MPMX20_01613 0.34 +1.4 1,707,067 - MPMX20_01613 0.34 +0.5 1,707,194 + MPMX20_01613 0.61 +0.1 1,707,194 - MPMX20_01613 0.61 -1.5 1,707,220 + MPMX20_01613 0.67 -1.2 1,707,220 + MPMX20_01613 0.67 -0.4 1,707,239 + MPMX20_01613 0.71 +4.1 1,707,293 - MPMX20_01613 0.83 +0.3 1,707,329 - -2.0 1,707,385 + -1.8 1,707,393 - +0.3 1,707,468 + -0.5 1,707,476 - +0.6 1,707,602 - MPMX20_01614 0.19 -1.6 1,707,628 + MPMX20_01614 0.22 +1.1 1,707,643 + MPMX20_01614 0.24 +0.0 1,707,709 - MPMX20_01614 0.32 -0.3 1,707,712 + MPMX20_01614 0.32 +0.3 1,707,770 - MPMX20_01614 0.39 +0.6 1,707,779 + MPMX20_01614 0.40 -0.5 1,707,827 + MPMX20_01614 0.46 -0.3 1,707,827 + MPMX20_01614 0.46 -0.7 1,707,835 - MPMX20_01614 0.47 -1.3 1,707,851 + MPMX20_01614 0.49 -1.0 1,707,878 + MPMX20_01614 0.53 -1.1 1,707,902 - MPMX20_01614 0.56 -1.4 1,707,951 - MPMX20_01614 0.62 -1.1 1,708,041 + MPMX20_01614 0.73 +0.3 1,708,191 - +0.3 1,708,210 - -0.3 1,708,255 - +1.0 1,708,302 - +0.5 1,708,347 - MPMX20_01615 0.15 -1.0
Or see this region's nucleotide sequence