Experiment: M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00818 and MPMX20_00819 are separated by 195 nucleotides MPMX20_00819 and MPMX20_00820 are separated by 101 nucleotides MPMX20_00820 and MPMX20_00821 are separated by 107 nucleotides
MPMX20_00818: MPMX20_00818 - Quinoprotein glucose dehydrogenase, at 860,453 to 862,843
_00818
MPMX20_00819: MPMX20_00819 - Hypoxanthine phosphoribosyltransferase, at 863,039 to 863,587
_00819
MPMX20_00820: MPMX20_00820 - Carbonic anhydrase 2, at 863,689 to 864,351
_00820
MPMX20_00821: MPMX20_00821 - putative ABC transporter ATP-binding protein YadG, at 864,459 to 865,385
_00821
Position (kb) 863
864
865 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9 at 862.693 kb on + strand at 862.713 kb on + strand at 862.787 kb on + strand at 862.795 kb on + strand at 862.808 kb on + strand at 862.857 kb on - strand at 862.945 kb on + strand at 862.947 kb on - strand at 862.947 kb on - strand at 862.950 kb on + strand at 862.953 kb on - strand at 862.958 kb on - strand at 862.973 kb on + strand at 862.997 kb on + strand at 863.005 kb on - strand at 863.196 kb on + strand, within MPMX20_00819 at 863.196 kb on + strand, within MPMX20_00819 at 863.201 kb on + strand, within MPMX20_00819 at 863.204 kb on - strand, within MPMX20_00819 at 863.204 kb on - strand, within MPMX20_00819 at 863.204 kb on - strand, within MPMX20_00819 at 863.216 kb on + strand, within MPMX20_00819 at 863.219 kb on + strand, within MPMX20_00819 at 863.224 kb on - strand, within MPMX20_00819 at 863.260 kb on + strand, within MPMX20_00819 at 863.374 kb on + strand, within MPMX20_00819 at 863.432 kb on - strand, within MPMX20_00819 at 863.445 kb on - strand, within MPMX20_00819 at 863.488 kb on + strand, within MPMX20_00819 at 863.565 kb on + strand at 863.565 kb on + strand at 863.588 kb on + strand at 863.588 kb on + strand at 863.620 kb on - strand at 863.620 kb on - strand at 863.670 kb on - strand at 863.699 kb on - strand at 863.735 kb on + strand at 863.775 kb on + strand, within MPMX20_00820 at 863.775 kb on + strand, within MPMX20_00820 at 863.815 kb on - strand, within MPMX20_00820 at 863.824 kb on + strand, within MPMX20_00820 at 863.826 kb on - strand, within MPMX20_00820 at 863.901 kb on + strand, within MPMX20_00820 at 863.952 kb on + strand, within MPMX20_00820 at 863.964 kb on + strand, within MPMX20_00820 at 863.998 kb on + strand, within MPMX20_00820 at 864.006 kb on - strand, within MPMX20_00820 at 864.101 kb on - strand, within MPMX20_00820 at 864.194 kb on + strand, within MPMX20_00820 at 864.204 kb on + strand, within MPMX20_00820 at 864.236 kb on - strand, within MPMX20_00820 at 864.290 kb on + strand at 864.294 kb on - strand at 864.333 kb on + strand at 864.341 kb on - strand at 864.345 kb on + strand at 864.371 kb on - strand at 864.372 kb on - strand at 864.391 kb on + strand at 864.391 kb on + strand at 864.399 kb on - strand at 864.399 kb on - strand at 864.425 kb on - strand at 864.450 kb on + strand at 864.475 kb on + strand at 864.475 kb on + strand at 864.483 kb on - strand at 864.483 kb on - strand at 864.685 kb on - strand, within MPMX20_00821 at 864.721 kb on + strand, within MPMX20_00821 at 864.729 kb on - strand, within MPMX20_00821 at 864.739 kb on - strand, within MPMX20_00821 at 864.927 kb on - strand, within MPMX20_00821 at 864.942 kb on - strand, within MPMX20_00821 at 864.981 kb on + strand, within MPMX20_00821 at 864.981 kb on + strand, within MPMX20_00821 at 864.989 kb on - strand, within MPMX20_00821 at 865.042 kb on + strand, within MPMX20_00821 at 865.042 kb on + strand, within MPMX20_00821 at 865.050 kb on - strand, within MPMX20_00821 at 865.119 kb on - strand, within MPMX20_00821 at 865.280 kb on + strand, within MPMX20_00821 at 865.288 kb on - strand, within MPMX20_00821 at 865.302 kb on - strand at 865.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031 remove 862,693 + +1.7 862,713 + -1.5 862,787 + +0.1 862,795 + +0.4 862,808 + -0.1 862,857 - -0.2 862,945 + +0.1 862,947 - -0.4 862,947 - +1.1 862,950 + -0.5 862,953 - -1.2 862,958 - +3.3 862,973 + -1.0 862,997 + -1.6 863,005 - -0.9 863,196 + MPMX20_00819 0.29 -0.9 863,196 + MPMX20_00819 0.29 +0.2 863,201 + MPMX20_00819 0.30 +1.7 863,204 - MPMX20_00819 0.30 -1.1 863,204 - MPMX20_00819 0.30 +2.3 863,204 - MPMX20_00819 0.30 -0.3 863,216 + MPMX20_00819 0.32 +0.6 863,219 + MPMX20_00819 0.33 +0.4 863,224 - MPMX20_00819 0.34 -1.1 863,260 + MPMX20_00819 0.40 -2.3 863,374 + MPMX20_00819 0.61 -0.2 863,432 - MPMX20_00819 0.72 +0.7 863,445 - MPMX20_00819 0.74 +0.2 863,488 + MPMX20_00819 0.82 -1.1 863,565 + -1.6 863,565 + +1.9 863,588 + -1.4 863,588 + -2.5 863,620 - -1.0 863,620 - -1.0 863,670 - -1.2 863,699 - -0.7 863,735 + -0.8 863,775 + MPMX20_00820 0.13 -1.0 863,775 + MPMX20_00820 0.13 -2.1 863,815 - MPMX20_00820 0.19 -0.4 863,824 + MPMX20_00820 0.20 -1.9 863,826 - MPMX20_00820 0.21 +0.0 863,901 + MPMX20_00820 0.32 -1.2 863,952 + MPMX20_00820 0.40 -0.5 863,964 + MPMX20_00820 0.41 -1.8 863,998 + MPMX20_00820 0.47 -1.3 864,006 - MPMX20_00820 0.48 -1.5 864,101 - MPMX20_00820 0.62 -0.5 864,194 + MPMX20_00820 0.76 -1.7 864,204 + MPMX20_00820 0.78 -1.3 864,236 - MPMX20_00820 0.83 -1.4 864,290 + -1.1 864,294 - -1.5 864,333 + +1.4 864,341 - +2.7 864,345 + -0.7 864,371 - -1.9 864,372 - -0.6 864,391 + -0.6 864,391 + +0.2 864,399 - -0.1 864,399 - +1.4 864,425 - -1.5 864,450 + +1.1 864,475 + -1.3 864,475 + +1.4 864,483 - -0.2 864,483 - -0.3 864,685 - MPMX20_00821 0.24 +0.1 864,721 + MPMX20_00821 0.28 -1.0 864,729 - MPMX20_00821 0.29 -0.0 864,739 - MPMX20_00821 0.30 +0.3 864,927 - MPMX20_00821 0.50 -0.1 864,942 - MPMX20_00821 0.52 -1.5 864,981 + MPMX20_00821 0.56 +3.7 864,981 + MPMX20_00821 0.56 -0.7 864,989 - MPMX20_00821 0.57 +0.9 865,042 + MPMX20_00821 0.63 -1.3 865,042 + MPMX20_00821 0.63 +0.0 865,050 - MPMX20_00821 0.64 -1.3 865,119 - MPMX20_00821 0.71 -0.2 865,280 + MPMX20_00821 0.89 -0.6 865,288 - MPMX20_00821 0.89 +0.1 865,302 - -1.2 865,348 + +9.5
Or see this region's nucleotide sequence