Experiment: M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00423 and MPMX20_00424 are separated by 137 nucleotides MPMX20_00424 and MPMX20_00425 are separated by 63 nucleotides MPMX20_00425 and MPMX20_00426 are separated by 40 nucleotides MPMX20_00426 and MPMX20_00427 are separated by 55 nucleotides MPMX20_00427 and MPMX20_00428 are separated by 108 nucleotides
MPMX20_00423: MPMX20_00423 - 60 kDa chaperonin, at 439,244 to 440,887
_00423
MPMX20_00424: MPMX20_00424 - hypothetical protein, at 441,025 to 441,378
_00424
MPMX20_00425: MPMX20_00425 - L-lysine 2,3-aminomutase, at 441,442 to 442,470
_00425
MPMX20_00426: MPMX20_00426 - Elongation factor P, at 442,511 to 443,077
_00426
MPMX20_00427: MPMX20_00427 - hypothetical protein, at 443,133 to 443,267
_00427
MPMX20_00428: MPMX20_00428 - hypothetical protein, at 443,376 to 443,522
_00428
Position (kb) 441
442
443 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 440.972 kb on + strand at 440.980 kb on - strand at 440.980 kb on - strand at 440.980 kb on - strand at 440.980 kb on - strand at 440.984 kb on + strand at 440.997 kb on + strand at 441.049 kb on - strand at 441.056 kb on - strand at 441.069 kb on - strand, within MPMX20_00424 at 441.069 kb on - strand, within MPMX20_00424 at 441.069 kb on - strand, within MPMX20_00424 at 441.069 kb on - strand, within MPMX20_00424 at 441.085 kb on - strand, within MPMX20_00424 at 441.097 kb on - strand, within MPMX20_00424 at 441.104 kb on + strand, within MPMX20_00424 at 441.107 kb on - strand, within MPMX20_00424 at 441.109 kb on + strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.117 kb on - strand, within MPMX20_00424 at 441.142 kb on + strand, within MPMX20_00424 at 441.216 kb on + strand, within MPMX20_00424 at 441.224 kb on - strand, within MPMX20_00424 at 441.236 kb on + strand, within MPMX20_00424 at 441.290 kb on - strand, within MPMX20_00424 at 441.326 kb on - strand, within MPMX20_00424 at 441.358 kb on + strand at 441.366 kb on - strand at 441.366 kb on - strand at 441.377 kb on + strand at 441.419 kb on + strand at 441.419 kb on + strand at 441.424 kb on + strand at 441.427 kb on - strand at 441.427 kb on - strand at 441.432 kb on - strand at 441.664 kb on - strand, within MPMX20_00425 at 442.032 kb on - strand, within MPMX20_00425 at 442.032 kb on - strand, within MPMX20_00425 at 442.185 kb on - strand, within MPMX20_00425 at 443.164 kb on + strand, within MPMX20_00427 at 443.169 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.174 kb on + strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.182 kb on - strand, within MPMX20_00427 at 443.195 kb on + strand, within MPMX20_00427 at 443.197 kb on + strand, within MPMX20_00427 at 443.203 kb on - strand, within MPMX20_00427 at 443.262 kb on + strand at 443.262 kb on + strand at 443.341 kb on + strand at 443.344 kb on - strand at 443.349 kb on - strand at 443.371 kb on + strand at 443.409 kb on + strand, within MPMX20_00428 at 443.436 kb on + strand, within MPMX20_00428 at 443.446 kb on - strand, within MPMX20_00428 at 443.446 kb on - strand, within MPMX20_00428 at 443.446 kb on - strand, within MPMX20_00428 at 443.463 kb on - strand, within MPMX20_00428
Per-strain Table
Position Strand Gene LocusTag Fraction M9 plate, 1:1 coculture with Microbacterium jejuense SAI_031 remove 440,972 + -1.3 440,980 - -0.3 440,980 - -1.1 440,980 - -1.6 440,980 - -0.8 440,984 + +2.2 440,997 + -0.0 441,049 - +0.1 441,056 - +0.3 441,069 - MPMX20_00424 0.12 -1.6 441,069 - MPMX20_00424 0.12 -0.6 441,069 - MPMX20_00424 0.12 -0.3 441,069 - MPMX20_00424 0.12 -0.8 441,085 - MPMX20_00424 0.17 +1.6 441,097 - MPMX20_00424 0.20 -1.2 441,104 + MPMX20_00424 0.22 -0.3 441,107 - MPMX20_00424 0.23 -2.9 441,109 + MPMX20_00424 0.24 +0.3 441,117 - MPMX20_00424 0.26 -1.3 441,117 - MPMX20_00424 0.26 -0.4 441,117 - MPMX20_00424 0.26 -1.6 441,117 - MPMX20_00424 0.26 +1.3 441,117 - MPMX20_00424 0.26 -0.7 441,117 - MPMX20_00424 0.26 +0.0 441,117 - MPMX20_00424 0.26 -0.4 441,142 + MPMX20_00424 0.33 -1.8 441,216 + MPMX20_00424 0.54 +1.3 441,224 - MPMX20_00424 0.56 -1.6 441,236 + MPMX20_00424 0.60 +1.0 441,290 - MPMX20_00424 0.75 -0.3 441,326 - MPMX20_00424 0.85 -1.4 441,358 + -1.1 441,366 - -1.6 441,366 - -1.2 441,377 + -0.2 441,419 + +1.0 441,419 + -0.9 441,424 + -0.2 441,427 - -3.0 441,427 - -0.4 441,432 - +0.3 441,664 - MPMX20_00425 0.22 +1.6 442,032 - MPMX20_00425 0.57 +0.6 442,032 - MPMX20_00425 0.57 +0.6 442,185 - MPMX20_00425 0.72 +1.0 443,164 + MPMX20_00427 0.23 -1.7 443,169 + MPMX20_00427 0.27 -1.3 443,174 + MPMX20_00427 0.30 -0.2 443,174 + MPMX20_00427 0.30 -0.5 443,174 + MPMX20_00427 0.30 +0.1 443,174 + MPMX20_00427 0.30 -2.4 443,174 + MPMX20_00427 0.30 -1.6 443,174 + MPMX20_00427 0.30 -0.4 443,174 + MPMX20_00427 0.30 -0.4 443,182 - MPMX20_00427 0.36 -0.4 443,182 - MPMX20_00427 0.36 -0.7 443,182 - MPMX20_00427 0.36 -1.0 443,182 - MPMX20_00427 0.36 -0.1 443,182 - MPMX20_00427 0.36 -1.3 443,195 + MPMX20_00427 0.46 -1.3 443,197 + MPMX20_00427 0.47 +5.7 443,203 - MPMX20_00427 0.52 -1.3 443,262 + +0.6 443,262 + +0.0 443,341 + -0.6 443,344 - +1.6 443,349 - +2.6 443,371 + +1.0 443,409 + MPMX20_00428 0.22 -1.2 443,436 + MPMX20_00428 0.41 -0.4 443,446 - MPMX20_00428 0.48 -0.9 443,446 - MPMX20_00428 0.48 +2.6 443,446 - MPMX20_00428 0.48 +1.0 443,463 - MPMX20_00428 0.59 -1.4
Or see this region's nucleotide sequence