Experiment: survival 4C, 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frdA and IJEDHG_07000 overlap by 8 nucleotides IJEDHG_07000 and frdC are separated by 11 nucleotides frdC and frdD overlap by 4 nucleotides frdD and IJEDHG_07015 are separated by 74 nucleotides
IJEDHG_06995: frdA - fumarate reductase (quinol) flavoprotein subunit, at 1,139,713 to 1,141,497
frdA
IJEDHG_07000: IJEDHG_07000 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 1,141,490 to 1,142,224
_07000
IJEDHG_07005: frdC - fumarate reductase subunit FrdC, at 1,142,236 to 1,142,640
frdC
IJEDHG_07010: frdD - fumarate reductase subunit FrdD, at 1,142,637 to 1,142,990
frdD
IJEDHG_07015: IJEDHG_07015 - Outer membrane lipoprotein Blc, at 1,143,065 to 1,143,589
_07015
Position (kb)
1142
1143 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1141.267 kb on - strand, within frdA at 1141.267 kb on - strand, within frdA at 1141.473 kb on + strand at 1141.474 kb on - strand at 1141.500 kb on + strand at 1141.511 kb on - strand at 1141.617 kb on - strand, within IJEDHG_07000 at 1141.637 kb on + strand, within IJEDHG_07000 at 1141.638 kb on - strand, within IJEDHG_07000 at 1141.638 kb on - strand, within IJEDHG_07000 at 1141.638 kb on - strand, within IJEDHG_07000 at 1141.703 kb on - strand, within IJEDHG_07000 at 1141.723 kb on + strand, within IJEDHG_07000 at 1141.723 kb on + strand, within IJEDHG_07000 at 1141.808 kb on - strand, within IJEDHG_07000 at 1141.847 kb on + strand, within IJEDHG_07000 at 1141.847 kb on + strand, within IJEDHG_07000 at 1141.847 kb on + strand, within IJEDHG_07000 at 1141.848 kb on - strand, within IJEDHG_07000 at 1141.848 kb on - strand, within IJEDHG_07000 at 1141.916 kb on + strand, within IJEDHG_07000 at 1141.939 kb on + strand, within IJEDHG_07000 at 1141.940 kb on - strand, within IJEDHG_07000 at 1142.029 kb on + strand, within IJEDHG_07000 at 1142.104 kb on + strand, within IJEDHG_07000 at 1142.105 kb on - strand, within IJEDHG_07000 at 1142.197 kb on + strand at 1142.197 kb on + strand at 1142.257 kb on + strand at 1142.258 kb on - strand at 1142.311 kb on + strand, within frdC at 1142.313 kb on + strand, within frdC at 1142.313 kb on + strand, within frdC at 1142.313 kb on + strand, within frdC at 1142.441 kb on + strand, within frdC at 1142.442 kb on - strand, within frdC at 1142.454 kb on + strand, within frdC at 1142.527 kb on - strand, within frdC at 1142.637 kb on - strand at 1142.746 kb on + strand, within frdD at 1142.747 kb on - strand, within frdD at 1142.759 kb on + strand, within frdD at 1142.784 kb on + strand, within frdD at 1142.784 kb on + strand, within frdD at 1142.969 kb on - strand at 1142.969 kb on - strand at 1142.989 kb on - strand at 1143.030 kb on - strand at 1143.032 kb on - strand at 1143.051 kb on + strand at 1143.064 kb on + strand at 1143.064 kb on + strand at 1143.065 kb on - strand at 1143.065 kb on - strand at 1143.128 kb on - strand, within IJEDHG_07015 at 1143.128 kb on - strand, within IJEDHG_07015 at 1143.145 kb on - strand, within IJEDHG_07015 at 1143.176 kb on + strand, within IJEDHG_07015 at 1143.177 kb on - strand, within IJEDHG_07015 at 1143.251 kb on + strand, within IJEDHG_07015 at 1143.251 kb on + strand, within IJEDHG_07015 at 1143.298 kb on + strand, within IJEDHG_07015 at 1143.299 kb on - strand, within IJEDHG_07015 at 1143.299 kb on - strand, within IJEDHG_07015 at 1143.299 kb on - strand, within IJEDHG_07015 at 1143.399 kb on - strand, within IJEDHG_07015 at 1143.466 kb on + strand, within IJEDHG_07015 at 1143.470 kb on + strand, within IJEDHG_07015 at 1143.470 kb on + strand, within IJEDHG_07015 at 1143.471 kb on - strand, within IJEDHG_07015 at 1143.471 kb on - strand, within IJEDHG_07015 at 1143.471 kb on - strand, within IJEDHG_07015 at 1143.511 kb on + strand, within IJEDHG_07015 at 1143.511 kb on + strand, within IJEDHG_07015 at 1143.514 kb on + strand, within IJEDHG_07015 at 1143.528 kb on + strand, within IJEDHG_07015
Per-strain Table
Position Strand Gene LocusTag Fraction survival 4C, 24 hrs remove 1,141,267 - frdA IJEDHG_06995 0.87 -1.6 1,141,267 - frdA IJEDHG_06995 0.87 +0.2 1,141,473 + +0.3 1,141,474 - +0.3 1,141,500 + -1.0 1,141,511 - +0.1 1,141,617 - IJEDHG_07000 0.17 -0.6 1,141,637 + IJEDHG_07000 0.20 +0.2 1,141,638 - IJEDHG_07000 0.20 -1.1 1,141,638 - IJEDHG_07000 0.20 -0.7 1,141,638 - IJEDHG_07000 0.20 +1.2 1,141,703 - IJEDHG_07000 0.29 +0.8 1,141,723 + IJEDHG_07000 0.32 +0.3 1,141,723 + IJEDHG_07000 0.32 +0.6 1,141,808 - IJEDHG_07000 0.43 +0.0 1,141,847 + IJEDHG_07000 0.49 +1.0 1,141,847 + IJEDHG_07000 0.49 +0.5 1,141,847 + IJEDHG_07000 0.49 +0.1 1,141,848 - IJEDHG_07000 0.49 +0.8 1,141,848 - IJEDHG_07000 0.49 +0.4 1,141,916 + IJEDHG_07000 0.58 +0.6 1,141,939 + IJEDHG_07000 0.61 -0.5 1,141,940 - IJEDHG_07000 0.61 -1.0 1,142,029 + IJEDHG_07000 0.73 -1.8 1,142,104 + IJEDHG_07000 0.84 +0.3 1,142,105 - IJEDHG_07000 0.84 -0.1 1,142,197 + +0.0 1,142,197 + -0.2 1,142,257 + +0.1 1,142,258 - -0.6 1,142,311 + frdC IJEDHG_07005 0.19 -0.8 1,142,313 + frdC IJEDHG_07005 0.19 +0.0 1,142,313 + frdC IJEDHG_07005 0.19 -1.0 1,142,313 + frdC IJEDHG_07005 0.19 -0.9 1,142,441 + frdC IJEDHG_07005 0.51 +0.6 1,142,442 - frdC IJEDHG_07005 0.51 -0.7 1,142,454 + frdC IJEDHG_07005 0.54 -0.6 1,142,527 - frdC IJEDHG_07005 0.72 +0.6 1,142,637 - -0.6 1,142,746 + frdD IJEDHG_07010 0.31 -0.0 1,142,747 - frdD IJEDHG_07010 0.31 -0.8 1,142,759 + frdD IJEDHG_07010 0.34 +0.0 1,142,784 + frdD IJEDHG_07010 0.42 -0.2 1,142,784 + frdD IJEDHG_07010 0.42 -0.6 1,142,969 - +1.1 1,142,969 - -1.0 1,142,989 - +0.2 1,143,030 - -1.6 1,143,032 - +0.6 1,143,051 + -2.1 1,143,064 + -1.0 1,143,064 + +0.1 1,143,065 - -1.0 1,143,065 - -0.6 1,143,128 - IJEDHG_07015 0.12 -0.2 1,143,128 - IJEDHG_07015 0.12 +0.3 1,143,145 - IJEDHG_07015 0.15 +0.2 1,143,176 + IJEDHG_07015 0.21 -0.2 1,143,177 - IJEDHG_07015 0.21 +1.1 1,143,251 + IJEDHG_07015 0.35 -0.6 1,143,251 + IJEDHG_07015 0.35 +0.0 1,143,298 + IJEDHG_07015 0.44 +0.6 1,143,299 - IJEDHG_07015 0.45 -0.3 1,143,299 - IJEDHG_07015 0.45 -0.1 1,143,299 - IJEDHG_07015 0.45 +0.4 1,143,399 - IJEDHG_07015 0.64 +1.8 1,143,466 + IJEDHG_07015 0.76 +0.4 1,143,470 + IJEDHG_07015 0.77 +0.7 1,143,470 + IJEDHG_07015 0.77 +0.3 1,143,471 - IJEDHG_07015 0.77 +0.2 1,143,471 - IJEDHG_07015 0.77 +0.3 1,143,471 - IJEDHG_07015 0.77 -1.1 1,143,511 + IJEDHG_07015 0.85 +0.8 1,143,511 + IJEDHG_07015 0.85 +0.3 1,143,514 + IJEDHG_07015 0.86 -0.3 1,143,528 + IJEDHG_07015 0.88 +1.4
Or see this region's nucleotide sequence